马蹄莲（马蹄莲属）上的褪绿斑点病由一种番茄斑萎病毒属病毒引起，该病毒在血清学上与西瓜银斑驳病毒相关，但亲缘关系较远。
A Chlorotic Spot Disease on Calla Lilies (Zantedeschia spp.) Is Caused by a Tospovirus Serologically but Distantly Related to Watermelon silver mottle virus.
作者信息
Chen C C, Chen T C, Lin Y H, Yeh S D, Hsu H T
机构信息
Department of Plant Protection, Taichung District Agricultural Improvement Station, Changhua, Taiwan.
Department of Plant Pathology, National Chung Hsing University, Taichung, 40227 Taiwan.
出版信息
Plant Dis. 2005 May;89(5):440-445. doi: 10.1094/PD-89-0440.
A new tospovirus, Calla lily chlorotic spot virus (CCSV), was isolated from calla lilies (Zantedeschia spp.) in Taiwan. Chlorotic spots, ranging from light green to yellow, appear on the middle leaves of the affected plants. Virions measuring 75 to 105 nm, similar in size to tospovirus particles, were present in crude extracts and ultrathin sections of diseased leaves. Of 35 plant species inoculated mechanically, 24, including wax gourd (Benincasa hispida) and zucchini squash (Cucurbita pepo), were susceptible to the virus. CCSV was transmitted from infected wax gourd by Thrips palmi to healthy wax gourd and zucchini squash. The virus was weakly related to Watermelon silver mottle virus (WSMoV) in enzyme-linked immunosorbent assay (ELISA) and western blot tests. WSMoV-specific N gene primers, however, failed to produce DNA fragments from total RNA extracts of CCSV-infected plants in reverse transcription-polymerase chain reaction (RT-PCR). Results of RT-PCR show that the conserved regions of the L genes of tospoviruses are present in CCSV.
一种新的番茄斑萎病毒属病毒——马蹄莲褪绿斑点病毒（CCSV），从台湾的马蹄莲（马蹄莲属植物）中分离得到。受感染植株的中部叶片上出现从浅绿色到黄色的褪绿斑点。在患病叶片的粗提物和超薄切片中存在大小为75至105纳米的病毒粒子，其大小与番茄斑萎病毒属粒子相似。在机械接种的35种植物中，包括冬瓜（冬瓜）和西葫芦（西葫芦）在内的24种植物对该病毒敏感。CCSV通过棕榈蓟马从受感染的冬瓜传播到健康的冬瓜和西葫芦。在酶联免疫吸附测定（ELISA）和蛋白质印迹试验中，该病毒与西瓜银斑驳病毒（WSMoV）的关系较弱。然而，WSMoV特异性N基因引物在逆转录-聚合酶链反应（RT-PCR）中未能从CCSV感染植株的总RNA提取物中产生DNA片段。RT-PCR结果表明，番茄斑萎病毒属病毒L基因的保守区域存在于CCSV中。
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