同时测定肉制品中的十二种染料：基于 HPLC-UV-二极管阵列检测的分析方法的建立与验证。
Simultaneous determination of twelve dyes in meat products: Development and validation of an analytical method based on HPLC-UV-diode array detection.
机构信息
National Reference Center for the Detection of Radioactivity in Feed and Foodstuff, Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Via Manfredonia 20, 71121 Foggia, Italy.
Department of the Sciences of Agriculture, Food and Environment, University of Foggia, Via Napoli, 25, 71122 Foggia, Italy.
出版信息
Food Chem. 2019 Jul 1;285:1-9. doi: 10.1016/j.foodchem.2019.01.133. Epub 2019 Jan 30.
The use of food dyes in meat is subject to regulations, due to food safety concerns. A reliable method for the determination of 12 food dyes (Amaranth, Ponceau 4R, Carmine, Ponceau SX, Ponceau 3R, Allura Red AC, Carmoisine, Erythrosine, Sudan I, Sudan II, Sudan III and Sudan IV) in meat products using high performance liquid chromatography coupled to UV-diode array detection was developed, optimized and fully validated. The extraction was accomplished using acetonitrile, methanol, water, ammonia, 50:40:9:1 (v/v/v/v) as the solvent, and an ultrasonic bath. Chromatographic separation was achieved using a C RP column and samples eluted with a gradient acetate-acetonitrile mobile phase. Good analytical performance was obtained, in terms of selectivity, sensitivity, accuracy and ruggedness. Both method precision (CV% range: 6.2%-18.0%) and recovery (range: 86.4%-105.0%) complied with Decision 657/2002/EC, suggesting the procedure could be applied successfully for analyses of meat products in the European Union.
由于食品安全问题，食品染料在肉类中的使用受到法规的限制。本研究建立并充分验证了一种使用高效液相色谱法结合紫外二极管阵列检测，用于测定肉制品中 12 种食品染料（苋菜红、诱惑红 4R、胭脂红、丽春红 SX、丽春红 3R、诱惑红 AC、喹啉黄、赤藓红、苏丹 I、苏丹 II、苏丹 III 和苏丹 IV）的可靠方法。采用乙腈、甲醇、水、氨水（50:40:9:1，v/v/v/v）作为溶剂，使用超声浴进行提取。采用 C₁₈色谱柱，梯度乙酸-乙腈流动相洗脱，实现了色谱分离。该方法在选择性、灵敏度、准确性和稳健性方面均具有良好的分析性能。方法精密度（CV%范围：6.2%-18.0%）和回收率（范围：86.4%-105.0%）均符合欧盟 657/2002/EC 号决定，表明该程序可成功应用于欧盟肉制品的分析。
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