α或γ天然干扰素、重组干扰素及干扰素诱导剂对自然杀伤细胞细胞毒性的增强作用。单核细胞的影响。

Augmentation of natural killer cytotoxicity by alpha or gamma natural and recombinant interferons and interferon inducers. Effect of monocytes.

作者信息

Platsoucas C D, Fernandes G, Good R A, Gupta S

出版信息

Int Arch Allergy Appl Immunol. 1986;79(1):1-7. doi: 10.1159/000233933.

Abstract

We investigated the effect of human peripheral blood monocytes on the augmentation of natural killer cytotoxicity by alpha or gamma natural and recombinant interferons (IFN) and certain interferon inducers. We observed that: (1) in the majority of the donors examined (75%) human peripheral blood monocytes do not affect natural killer cytotoxicity, determined by a 4-hour chromium-51 release assay, against target cells from hemopoietic human tumor cell lines. (2) Monocytes are not required and do not affect the augmentation of natural killer cytotoxicity by Escherichia coli-derived IFN-gamma, natural human IFN-gamma, E. Coli-derived IFN-alpha 2 or natural human IFN-alpha. E. Coli-derived IFN-gamma and natural human IFN-gamma have been reported to activate monocyte cytotoxicity determined in 72-hour assay. (3) Monocytes are not required for the augmentation of natural killer cytotoxicity against target cells from hemopoietic tumor cell lines by polyinosinic acid-polycytidylic acid or staphylococcal enterotoxin A.

摘要

我们研究了人类外周血单核细胞对α或γ天然及重组干扰素（IFN）以及某些干扰素诱导剂增强自然杀伤细胞细胞毒性作用的影响。我们观察到：（1）在大多数接受检测的供体（75%）中，通过4小时的铬-51释放试验测定，人类外周血单核细胞对来自人类造血肿瘤细胞系的靶细胞的自然杀伤细胞细胞毒性没有影响。（2）单核细胞并非增强自然杀伤细胞细胞毒性所必需，且不影响大肠杆菌来源的IFN-γ、天然人IFN-γ、大肠杆菌来源的IFN-α2或天然人IFN-α对自然杀伤细胞细胞毒性的增强作用。据报道，大肠杆菌来源的IFN-γ和天然人IFN-γ可激活在72小时试验中测定的单核细胞细胞毒性。（3）对于多聚肌苷酸-多聚胞苷酸或葡萄球菌肠毒素A增强针对来自造血肿瘤细胞系的靶细胞的自然杀伤细胞细胞毒性而言，单核细胞并非必需。