一种使用平板读数仪进行血凝抑制试验的数字数据解读方法。
A digital data interpretation method for hemagglutination inhibition assay by using a plate reader.
机构信息
US Horticultural Research Laboratory, 2001 South Rock Road, Fort Pierce, FL, 34945, USA.
US Horticultural Research Laboratory, 2001 South Rock Road, Fort Pierce, FL, 34945, USA.
出版信息
Anal Biochem. 2019 Apr 15;571:37-39. doi: 10.1016/j.ab.2019.02.016. Epub 2019 Feb 21.
Hemagglutination inhibition (HAI) assay is a simple method quantifying relative binding activities of glycan-lectin interactions. Currently, interpretation of HAI data remains a manual task depending on visual observation. In this study we developed a digital data reading method for HAI assay by using the area scanning function of a microplate reader. This was based on galectin-3-induced hemagglutination inhibition assay. OD values showed a four-parameter logistic correlation with concentrations of galectin-3 inhibitors (R ≥ 0.97), and IC values were obtained from the curve fitting. The method provides an objective and robust data interpretation for HAI assays conducted with chicken erythrocytes.
血凝抑制（HAI）试验是一种定量测定糖-凝集素相互作用相对结合活性的简单方法。目前，HAI 数据的解释仍然是一项依赖于视觉观察的手动任务。在这项研究中，我们通过使用微孔板读数器的面积扫描功能，开发了一种用于 HAI 试验的数字数据读取方法。这是基于半乳糖凝集素-3 诱导的血凝抑制试验。OD 值与半乳糖凝集素-3 抑制剂的浓度呈四参数逻辑相关（R≥0.97），并通过曲线拟合得到 IC 值。该方法为使用鸡红细胞进行的 HAI 试验提供了客观、稳健的数据解释。
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