Wilharm Gottfried, Skiebe Evelyn
Robert Koch Institute, Wernigerode, Germany.
Methods Mol Biol. 2019;1946:75-85. doi: 10.1007/978-1-4939-9118-1_8.
The genomes of Acinetobacter baumannii tell us stories about horizontal gene transfer (HGT) events that steadily drive the evolution of this nosocomial pathogen toward multidrug resistance. Natural transformation competence constitutes one of the several possible pathways that mediate HGT in A. baumannii. Here, we describe and discuss the methods for studying DNA uptake in A. baumannii via natural transformation.
鲍曼不动杆菌的基因组向我们讲述了水平基因转移（HGT）事件的故事，这些事件稳步推动这种医院病原体朝着多重耐药性进化。自然转化能力是介导鲍曼不动杆菌中HGT的几种可能途径之一。在这里，我们描述并讨论了通过自然转化研究鲍曼不动杆菌中DNA摄取的方法。
