Roumen Tsanev Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Roumen Tsanev Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia, Bulgaria.
Adv Protein Chem Struct Biol. 2019;115:69-94. doi: 10.1016/bs.apcsb.2018.11.003. Epub 2018 Dec 20.
DNA double strand breaks (DSB) are the most deleterious type of damage inflicted on DNA by various environmental factors and as consequences of normal cellular metabolism. The multistep nature of DSB repair and the need to assemble large protein complexes at repair sites necessitate multiple chromatin changes there. This review focuses on the key findings of how chromatin regulators exert temporal and spatial control on DSB repair. These mechanisms coordinate repair with cell cycle progression, lead to DSB repair pathway choice, provide accessibility of repair machinery to damaged sites and move the lesions to nuclear environments permissive for repair.
DNA 双链断裂（DSB）是各种环境因素和正常细胞代谢导致的 DNA 最具危害性的损伤类型。DSB 修复的多步骤性质以及在修复部位组装大型蛋白质复合物的需要，需要在那里进行多次染色质改变。本综述重点介绍了染色质调节因子如何对 DSB 修复进行时空控制的关键发现。这些机制协调修复与细胞周期进程，导致 DSB 修复途径的选择，为修复机器提供受损部位的可及性，并将病变转移到有利于修复的核环境中。
