Yuasa Hideto, Mantani Youhei, Miyamoto Kazuki, Nishida Miho, Arai Masaya, Tsuruta Hiroki, Yokoyama Toshifumi, Hoshi Nobuhiko, Kitagawa Hiroshi
Department of Anatomy and Regenerative Biology, Graduate School of Medicine, Osaka City University, 1-4-3 Asahi-machi, Abeno-ku, Osaka 545-8585, Japan.
Laboratory of Histophysiology, Department of Bioresource Science, Graduate School of Agricultural Science, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, Japan.
J Vet Med Sci. 2019 Apr 16;81(4):555-566. doi: 10.1292/jvms.18-0734. Epub 2019 Feb 25.
The effect of bacterial colonies expanded into the intervillous spaces on the localization of several lymphocyte lineages was immunohistochemically investigated in two types of mucosa: ordinary mucosa of rat ileum, which consists of mucosa without any mucosal lymphatic tissue; and follicle-associated mucosa (FAM), which accompanies the parafollicular area under the muscularis mucosae in the rat ileal Peyer's patch. The results showed that bacterial colonies in the intervillous spaces induced increased populations of CD8 cells in the epithelium of the intestinal villus in ordinary mucosa (IV) and intestinal villus in FAM (IV-FAM). Bacterial colonies in the intervillous spaces were also associated with increased numbers of IgA cells, which were mainly localized in the lamina propria of basal portions of IV and IV-FAM, and with expanded localization of IgA cells into the villous apex in both IV and IV-FAM. Moreover, IgA cells around the intestinal crypts adjacent to IV or IV-FAM were also increased in response to bacterial colonies. In the IV-FAM, but not IV, L-selectin cells, which were found to be immunopositive for TCRαβ or CD19, were drastically increased in the lamina propria from the crypt to middle portion of IV-FAM and in the lumen of central lymph vessel of IV-FAM in response to the bacterial colonies in the intervillous spaces. These findings revealed that the expansion of bacterial colonies into the intervillous spaces accompanies the change of histological localization of the lymphocyte lineage in both the ordinary mucosa and FAM.
采用免疫组织化学方法，研究了扩展至绒毛间隙的细菌菌落对两种类型黏膜中几种淋巴细胞谱系定位的影响：一种是大鼠回肠的普通黏膜，由无任何黏膜淋巴组织的黏膜组成；另一种是滤泡相关黏膜（FAM），它伴随着大鼠回肠派伊尔结黏膜肌层下的滤泡旁区域。结果显示，绒毛间隙中的细菌菌落导致普通黏膜（IV）和FAM的肠绒毛（IV - FAM）上皮中CD8细胞数量增加。绒毛间隙中的细菌菌落还与IgA细胞数量增加有关，这些细胞主要定位于IV和IV - FAM基部的固有层，并且在IV和IV - FAM中，IgA细胞向绒毛顶端的定位也有所扩展。此外，与IV或IV - FAM相邻的肠隐窝周围的IgA细胞也因细菌菌落而增加。在IV - FAM而非IV中，对TCRαβ或CD19呈免疫阳性的L - 选择素细胞，在IV - FAM从隐窝到中部的固有层以及IV - FAM中央淋巴管腔中，因绒毛间隙中的细菌菌落而急剧增加。这些发现表明，细菌菌落扩展至绒毛间隙伴随着普通黏膜和FAM中淋巴细胞谱系组织学定位的变化。
