对乙酰氨基酚诱导大鼠肝炎的抗炎和抗氧化作用。
Anti-inflammatory and Antioxidant Effects of on Paracetamol-induced Hepatitis in Rats.
机构信息
Laboratory of Biology and Environment, Faculty of Nature and Life Sciences, University of Mentouri Brothers, Constantine, Algeria.
VARENBIOMOL Research Unit, University of Mentouri Brothers, Constantine, Algeria.
出版信息
Antiinflamm Antiallergy Agents Med Chem. 2020;19(2):128-139. doi: 10.2174/1871523018666190222120752.
BACKGROUND
Herbal medicines have been used in the treatment of liver diseases for a long time. The current study was elaborated to evaluate in vitro and in vivo antioxidant and anti-inflammatory effects of Lotus corniculatus (L. corniculatus) butanolic extract.
METHODS
The in vitro antioxidant and anti-inflammatory properties of L. corniculatus were investigated by employing DPPH radical scavenging, H2O2 scavenging and BSA denaturation assays. In vivo antioxidant and anti-inflammatory effects of L. corniculatus were evaluated against paracetamol (APAP)-induced hepatitis in rats. L.corniculatus at doses of 100 and 200 mg/kg was administered orally once daily for seven days. Serum transaminases (AST and ALT) and lactate dehydrogenase (LDH), total bilirubin levels, liver malondialdehyde (MDA), reduced glutathione (GSH), glutathione S- transferase (GST) and superoxide dismutase (SOD) levels and inflammatory markers, such as serum Creactive protein (CRP), circulating and liver myeloperoxidase (MPO) levels were investigated. Further histopathological analysis of the liver sections was performed to support the effectiveness of L. corniculatus.
RESULTS
L. corniculatus exhibited strong antioxidant and anti-inflammatory effects in vitro. In the in vivo study, our findings demonstrate that L. corniculatus (100 and 200 mg/kg) administration led to an amelioration of APAP effects on liver histology, liver functions parameters (AST, ALT, LDH, and total bilirubin levels) and liver oxidative stress markers (MDA, GSH, GST and SOD levels). Furthermore, serum CRP, circulating MPO and liver MPO levels were declined by both doses of L. corniculatus extract. The best benefits were observed with 200 mg/kg of L. corniculatus extract.
CONCLUSION
Antioxidant and anti-inflammatory effects of L. corniculatus extract may be due to the presence of active components.
背景
草药在治疗肝脏疾病方面已有很长的应用历史。本研究旨在详细评估莲须（Lotus corniculatus）正丁醇提取物的体外和体内抗氧化和抗炎作用。
方法
采用 DPPH 自由基清除、H2O2 清除和 BSA 变性测定法，研究莲须的体外抗氧化和抗炎特性。采用对乙酰氨基酚（APAP）诱导的大鼠肝炎模型评价莲须的体内抗氧化和抗炎作用。莲须以 100 和 200 mg/kg 的剂量每天口服给药一次，共 7 天。检测血清转氨酶（AST 和 ALT）和乳酸脱氢酶（LDH）、总胆红素水平、肝丙二醛（MDA）、还原型谷胱甘肽（GSH）、谷胱甘肽 S-转移酶（GST）和超氧化物歧化酶（SOD）水平以及炎症标志物，如血清 C 反应蛋白（CRP）、循环和肝髓过氧化物酶（MPO）水平。进一步进行肝组织切片的组织病理学分析以支持莲须的有效性。
结果
莲须在体外表现出很强的抗氧化和抗炎作用。在体内研究中，我们的研究结果表明，莲须（100 和 200 mg/kg）给药可改善 APAP 对肝脏组织学、肝功能参数（AST、ALT、LDH 和总胆红素水平）和肝氧化应激标志物（MDA、GSH、GST 和 SOD 水平）的影响。此外，两种剂量的莲须提取物均可降低血清 CRP、循环 MPO 和肝 MPO 水平。200 mg/kg 莲须提取物的效果最佳。
结论
莲须提取物的抗氧化和抗炎作用可能是由于其含有活性成分。
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