Morrison G, Cunningham-Rundles S, Clowes G H, Stahl W M
Ann Surg. 1986 Jan;203(1):21-4. doi: 10.1097/00000658-198601000-00004.
Natural killer cell (NK) activity was assessed in patients with nonthermal injury. NK activity was assessed employing a standard 4-hour 51Cr release. Peripheral mononuclear cells from healthy human donors and trauma ward patients served as effector cells at three effector/target ratios. Labeled K562 erythroleukemia cells were used as targets. Multiple dilutions of crude and purified proteolysis inducing factor (PIF), isolated from three septic patients, were evaluated in comparison with other adjuvants. Greater augmentation of anti-K562 activity was obtained in long-term pretreatment of effector cells with adjuvants present as opposed to immediate NK assay. Untreated effectors from trauma patients demonstrated the least amount of baseline NK activity; however, a combination of PIF and interferon showed the greatest cytotoxicity. Untreated healthy cells exhibited a significant (p less than 0.001), twofold greater amount of cytotoxicity than untreated trauma cells. PIF enhances NK activity and may be key to mobilization of host defense in trauma.
对非热损伤患者的自然杀伤细胞（NK）活性进行了评估。采用标准的4小时51Cr释放法评估NK活性。来自健康人类供体和创伤病房患者的外周血单个核细胞在三种效应细胞/靶细胞比例下用作效应细胞。标记的K562红白血病细胞用作靶细胞。与其他佐剂相比，评估了从三名脓毒症患者中分离出的粗制和纯化的蛋白水解诱导因子（PIF）的多种稀释液。与立即进行NK检测相反，用存在的佐剂对效应细胞进行长期预处理可获得更大的抗K562活性增强。创伤患者未经处理的效应细胞表现出最少的基线NK活性；然而，PIF和干扰素的组合显示出最大的细胞毒性。未经处理的健康细胞表现出显著（p小于0.001）的细胞毒性，比未经处理的创伤细胞高出两倍。PIF可增强NK活性，可能是创伤中宿主防御动员的关键。
