Ferhat L, Chahed A, Hamrouche S, Korichi-Ouar M, Hamdi T-M
Laboratory of Food Hygiene and Quality Insurance System, High National Veterinary School of Algiers, Algiers, Algeria.
High National Veterinary School of Algiers, Algiers, Algeria.
Lett Appl Microbiol. 2019 Jun;68(6):546-552. doi: 10.1111/lam.13142. Epub 2019 Mar 19.
Domestic ruminants are regarded as the major reservoir of Shiga toxin-producing Escherichia coli (STEC) closely related to human infection. A total of 363 ovine carcasses were swabbed in an Algiers city slaughterhouse for research on STEC. First of all, screening of the STECs was carried out by a multiplex PCR searching for the genes coding for the virulence factors stx , stx and eae. This step was followed by STEC isolation and serotyping. The presence of stx /stx eae genes was shown in 116 sheep carcasses (31·95%). From the 116 positive samples, 20 bacterial strains (17·24%) were isolated. Nineteen strains belonged to the species E. coli (STEC), and 1 belonged to Citrobacter braakii (eae stx ). During this study, the presence of potentially pathogenic STEC for humans on the surface of sheep carcasses was confirmed. Corrective measures should be considered at the slaughterhouse level to avoid outbreaks of STEC in Algeria. SIGNIFICANCE AND IMPACT OF THE STUDY: PCR screening revealed the significant presence of the genetic markers of Shiga toxin-producing Escherichia coli (STEC) (stx /stx eae ) on the surfaces of sheep carcasses. Citrobacter braakii (stx eae ) was isolated for the first time in this study. The risk of foodborne diseases due to STEC must be taken into account in Algeria. To prevent the emergence of epidemic outbreaks among children and older by people, preventive measures should be taken.
家养反刍动物被视为与人类感染密切相关的产志贺毒素大肠杆菌（STEC）的主要宿主。在阿尔及尔市的一家屠宰场，共采集了363份羊胴体样本用于STEC研究。首先，通过多重PCR对STEC进行筛查，寻找编码毒力因子stx、stx和eae的基因。此步骤之后是STEC的分离和血清分型。116份羊胴体样本（31.95%）中检测到stx/stx eae基因的存在。从这116份阳性样本中，分离出20株细菌菌株（17.24%）。其中19株属于大肠杆菌（STEC），1株属于布氏柠檬酸杆菌（eae stx）。在本研究中，证实了羊胴体表面存在对人类有潜在致病性的STEC。应在屠宰场层面考虑采取纠正措施，以避免阿尔及利亚爆发STEC疫情。研究的意义和影响：PCR筛查显示，羊胴体表面产志贺毒素大肠杆菌（STEC）（stx/stx eae）的遗传标记大量存在。本研究首次分离出布氏柠檬酸杆菌（stx eae）。阿尔及利亚必须考虑由STEC引起食源性疾病的风险。为防止儿童和老年人中出现疫情爆发，应采取预防措施。
