SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Alice, South Africa.
Molecular Pathogenesis and Molecular Epidemiology Research Group, Department of Biochemistry and Microbiology, University of Fort Hare, Alice, South Africa.
Microbiologyopen. 2019 Mar;8(3):e00449. doi: 10.1002/mbo3.449. Epub 2019 Feb 23.
In this study, we investigated the diversity of drug-resistant Mycobacterium tuberculosis isolates from families who own cattle in the Eastern Cape Province of South Africa using spoligotyping and mycobacterial interspersed repetitive-unit-variable number tandem repeat (MIRU-VNTR) typing. The Mycobacterium tuberculosis was investigated using MIRU-VNTR and the Mycobacterium tuberculosis families were evaluated using spoligotyping. Spoligotyping grouped 91% of the isolates into seven clusters, while 9% of the deoxyribonucleic acid (DNA) from TB isolates were unclustered from a total of 154 DNA used. Previously described shared types were observed in 89.6% of the isolates, with the Beijing family, SIT1, the principal genotype in the province, while the families T, SIT53 and X1, SIT1329 were the least detected genotypes. MIRU-VNTR grouped 81% of the isolates in 23 clusters while 19% were unclustered. A combination of the VNTR and spoligotyping grouped 79% of the isolates into 23 clusters with 21% unclustered. The low level of diversity and the clonal spread of drug-resistant Mycobacterium tuberculosis isolates advocate that the spread of TB in this study may be instigated by the clonal spread of Beijing genotype. The results from this study provide vital information about the lack of TB control and distribution of Mycobacterium tuberculosis complex strain types in the Eastern Cape Province of South Africa.
在这项研究中，我们使用 spoligotyping 和分枝杆菌间隔重复单元可变数量串联重复（MIRU-VNTR）分型技术，调查了南非东开普省拥有牛的家庭中耐药结核分枝杆菌分离株的多样性。使用 MIRU-VNTR 对结核分枝杆菌进行了研究，并用 spoligotyping 对结核分枝杆菌家族进行了评估。 spoligotyping 将 91%的分离株分为 7 个群，而总共使用的 154 个 DNA 中有 9%的 TB 分离株 DNA 未聚类。在 89.6%的分离株中观察到先前描述的共享类型，其中以北京家族、SIT1 为主导基因型，而 T、SIT53 和 X1、SIT1329 家族则是检测到的最少的基因型。MIRU-VNTR 将 81%的分离株分为 23 个群，而 19%的分离株未聚类。VNTR 和 spoligotyping 的组合将 79%的分离株分为 23 个群，其中 21%的分离株未聚类。耐药结核分枝杆菌分离株的低多样性和克隆传播表明，本研究中结核病的传播可能是由北京基因型的克隆传播引发的。本研究的结果提供了有关南非东开普省结核病控制和结核分枝杆菌复合菌株类型分布缺乏的重要信息。
