Kurnick J T, Kradin R L, Blumberg R, Schneeberger E E, Boyle L A
Clin Immunol Immunopathol. 1986 Mar;38(3):367-80. doi: 10.1016/0090-1229(86)90247-3.
Tissue fragments from biopsies of six patients with malignant tumors of the lung were cultured in interleukin 2 (IL-2). Cultures of proliferating lymphocytes were isolated from all cases. Tumor cell lines (small cell carcinoma and adenocarcinoma) were established in parallel cultures from two of these patients. Lymphocytes that proliferated in vitro were virtually all mature T lymphocytes (greater than 95% T3+, T11+). The T8+ subset accounted for an average of 70% while T4+ cells averaged 20% of the cells in culture. HNK-1 antigen was presented on 23% of cells. Seventy-four percent of cells expressed Ia (HLA-DR) antigens. B cells did not proliferate under these conditions. In all cases the cells lysed K562 targets and were active in lectin-mediated cytolysis against human lymphoblasts. All cultures produced lymphokines (IL-2 and IFN-gamma) when stimulated with PHA. Lymphocytes grown from a tissue specimen with adenocarcinoma were capable of killing autologous tumor cells in vitro. Specific cytotoxicity has been maintained by these cultured lymphocytes for greater than 6 months. IL-2 activated peripheral blood cells in this case showed little specific cytotoxicity for autologous tumor cells. Lymphocytes from another specimen of adenocarcinoma also lysed this tumor, but cells from the other four specimens did not. Lymphocytes propagated from the specimen of small cell undifferentiated cancer did not lyse autologous tumor cells. These data show that primary lung tumors contain activated T cells which will respond to IL-2 in vitro. These tumor-infiltrating lymphocytes have demonstrable function, which can include cytolytic activity against autologous lung tumor.
取6例肺癌患者活检的组织碎片，在白细胞介素2（IL-2）中进行培养。所有病例均分离出增殖淋巴细胞培养物。从其中2例患者的平行培养物中建立了肿瘤细胞系（小细胞癌和腺癌）。体外增殖的淋巴细胞几乎全是成熟T淋巴细胞（T3+、T11+大于95%）。T8+亚群平均占培养细胞的70%，而T4+细胞平均占20%。23%的细胞表达HNK-1抗原。74%的细胞表达Ia（HLA-DR）抗原。在这些条件下B细胞不增殖。所有病例的细胞均能裂解K562靶细胞，并在凝集素介导的对人淋巴母细胞的细胞溶解中具有活性。用PHA刺激时，所有培养物均产生淋巴因子（IL-2和IFN-γ）。从腺癌组织标本中培养出的淋巴细胞能够在体外杀伤自体肿瘤细胞。这些培养的淋巴细胞已保持特异性细胞毒性超过6个月。在这种情况下，IL-2激活的外周血细胞对自体肿瘤细胞几乎没有特异性细胞毒性。来自另一腺癌标本的淋巴细胞也能裂解该肿瘤，但来自其他4个标本的细胞则不能。从未分化小细胞癌标本中培养出的淋巴细胞不能裂解自体肿瘤细胞。这些数据表明，原发性肺肿瘤含有活化的T细胞，它们在体外对IL-2有反应。这些肿瘤浸润淋巴细胞具有可证实的功能，其中可包括对自体肺肿瘤的细胞溶解活性。
