Ireland M E, Rosenblum B B, Welsh M J
Endocrinology. 1986 Feb;118(2):526-32. doi: 10.1210/endo-118-2-526.
The response of cultured Sertoli cells to short term FSH stimulation was studied to elucidate early events involved in the hormone response of this cell type. The phosphorylation of proteins by [32P]orthophosphate-labeled cells was examined using two-dimensional polyacrylamide gel electrophoresis and autoradiography. FSH stimulation resulted in a variety of changes in the phosphoprotein labeling pattern. Within 5 min, unique phosphoproteins appeared in autoradiograms of treated cells. Increased labeling of vimentin was also noted. After 25 min of FSH stimulation, increases and decreases in apparent labeling intensity were evident in additional phosphoproteins that were constitutively labeled in control cultures. Changes in protein patterns in stained gels were also noted after acute hormone treatment. These observations demonstrate that Sertoli cells respond to hormonal stimulation in a detectable manner within 5 min. Cytoskeletal involvement in initial phases of hormone response is indicated. Mechanisms such as increased protein kinase activity, changes in protein kinase substrate affinity, increased protein turnover, or phosphatase activation may all contribute to the early events involved in Sertoli cell hormone response.
为了阐明培养的支持细胞对该激素反应所涉及的早期事件，研究了培养的支持细胞对短期促卵泡激素（FSH）刺激的反应。使用二维聚丙烯酰胺凝胶电泳和放射自显影法检测了用[32P]正磷酸盐标记的细胞中蛋白质的磷酸化情况。FSH刺激导致磷蛋白标记模式发生多种变化。在5分钟内，处理过的细胞的放射自显影片中出现了独特的磷蛋白。波形蛋白的标记也增加了。FSH刺激25分钟后，在对照培养物中组成性标记的其他磷蛋白中，明显的标记强度有增加和减少。急性激素处理后，染色凝胶中的蛋白质模式也有变化。这些观察结果表明，支持细胞在5分钟内以可检测的方式对激素刺激作出反应。表明细胞骨架参与了激素反应的初始阶段。诸如蛋白激酶活性增加、蛋白激酶底物亲和力变化、蛋白质周转增加或磷酸酶激活等机制都可能导致支持细胞激素反应所涉及的早期事件。
