Trigg M E, Peterson A, Erickson C, Bozdech M, Billing R, Sondel P, Finlay J, Surfus J, Stuiber M, Hong R
Exp Hematol. 1986 Jan;14(1):21-6.
T lymphocytes were depleted from donor marrow for 23 patients undergoing allogeneic bone marrow transplantation using an anti-T-cell antibody, CT-2, and complement. The methodology is described in detail for in vitro depletion of large quantities of bone marrow. The extent of T-lymphocyte depletion using various T-cell markers, the percent of marrow lost in the processing and quantity of antibody, and complement needed are presented. These techniques for in vitro T-lymphocyte depletion were reproducible and did result in an average final yield of 47% of the harvested donor marrow.
