Gamcsik M P, Gerig J T
FEBS Lett. 1986 Feb 3;196(1):71-4. doi: 10.1016/0014-5793(86)80216-2.
Rabbits ingesting 4-fluorophenylalanine are known to incorporate small amounts of this fluorinated amino acid into their proteins. Carbonic anhydrase I isolated from the erythrocytes of animals so maintained exhibits a well-resolved fluorine NMR signal for each phenylalanine in the sequence. The chemical shifts of most of these signals respond to the binding of inhibitors, suggesting that most if not all of the tertiary structure of the enzyme adjusts to the presence of inhibitors at the active site.
已知摄入4-氟苯丙氨酸的兔子会将少量这种氟化氨基酸掺入其蛋白质中。从以这种方式饲养的动物的红细胞中分离出的碳酸酐酶I，其序列中的每个苯丙氨酸都表现出分辨率良好的氟核磁共振信号。这些信号中的大多数化学位移会对抑制剂的结合产生反应，这表明该酶的三级结构大部分（如果不是全部的话）会根据活性位点处抑制剂的存在进行调整。
