接受放疗或放化疗的头颈癌患者的内皮微粒与血管内皮生长因子
Endothelial Microparticles and Vascular Endothelial Growth Factor in Patients With Head and Neck Cancer Undergoing Radiotherapy or Radiochemotherapy.
作者信息
Sierko Ewa, Sobierska Monika, Zabrocka Ewa, Kruszewska Joanna, Myśliwiec Marta, Lipska Alina, Radziwon Piotr, Wojtukiewicz Marek Z
机构信息
Department of Oncology, Medical University of Bialystok, Bialystok, Poland
Department of Radiotherapy, Comprehensive Cancer Center in Bialystok, Bialystok, Poland.
出版信息
In Vivo. 2019 Mar-Apr;33(2):581-586. doi: 10.21873/invivo.11514.
BACKGROUND
Endothelial microparticles (EMPs) released from activated or apoptotic endothelial cells may play a role in coagulation and thrombus formation. However, there is insufficient evidence regarding the impact of EMPs on angiogenesis in patients with cancer.
MATERIALS AND METHODS
Sixteen patients with head and neck cancer (HNC) undergoing radiotherapy/radiochemotherapy (RT/RCT) and 10 healthy controls were studied. Serum EMPs were counted by flow cytometry, and vascular endothelial growth factor (VEGF) was measured by enzyme-linked immunosorbent assay (ELISA).
RESULTS
The mean EMP level was significantly higher in patients with HNC before RT/RCT (1,601±1,479 EMP/μl) compared to the control group (782±698 EMP/μl). The number of EMPs was not notably increased after RT/RCT (1,629±769 EMP/μl). There was no significant correlation between the plasma EMP number and concentration of VEGF before (r=0.131; p=0.625), 1 day after (r=-0.042, p=0.874), nor 3 months after RT/RCT (r=0.454, p=0.076).
CONCLUSION
Released EMPs may not influence promotion of neovascularization in patients with HNC.
背景
从活化或凋亡的内皮细胞释放的内皮微粒（EMPs）可能在凝血和血栓形成中起作用。然而，关于EMPs对癌症患者血管生成的影响，证据不足。
材料与方法
研究了16例接受放疗/放化疗（RT/RCT）的头颈癌（HNC）患者和10名健康对照者。通过流式细胞术计数血清EMPs，并通过酶联免疫吸附测定（ELISA）测量血管内皮生长因子（VEGF）。
结果
与对照组（782±698个EMPs/μl）相比，HNC患者在RT/RCT前的平均EMPs水平显著更高（1,601±1,479个EMPs/μl）。RT/RCT后EMPs数量没有显著增加（1,629±769个EMPs/μl）。在RT/RCT前（r = 0.131；p = 0.625）、1天后（r = -0.042，p = 0.874）或3个月后（r = 0.454，p = 0.076），血浆EMPs数量与VEGF浓度之间均无显著相关性。
结论
释放的EMPs可能不会影响HNC患者新生血管形成的促进作用。
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