Department of Cardiology, Shanghai 9th People's Hospital, Shanghai Jiaotong University Medical School.
Circ J. 2014;78(1):206-14. doi: 10.1253/circj.cj-13-0488. Epub 2013 Nov 8.
Endothelial microparticles (EMPs) are vehicles released from activated or apoptotic endothelium. The aim of this study was to establish a new cytometric bead assay for EMPs and investigate the prognostic value of EMPs in chest pain patients.
We invented and verified the cytometric bead assay to quantify EMP level in vitro. A total of 80 healthy volunteers and 350 chest pain patients were recruited and the EMPs measured. The major adverse cardiovascular events (MACE) of documented coronary artery disease patients were recorded in the follow-up period. The level of EMPs statistically correlated with those of endothelin-1 (ET-1) and intercellular adhesion molecule-1 (ICAM-1) in vitro. The EMP level in healthy subjects was <300.10. The patients had a remarkably higher EMP level than healthy subjects. Diabetes mellitus, EMP, and ET-1 levels were significantly associated with future cardiovascular events in chest pain patients. There was a significantly higher event incidence in the top tertile EMP level than in the lower tertile in the acute coronary syndrome (ACS) patient group.
A novel EMP quantification assay has been successfully established. The EMPs in vitro and in patients were significantly correlated with ET-1 and ICAM-1 level. The patients with a higher EMP level had a higher risk of MACE. EMP level is a predictor for MACE in ACS patients.
内皮细胞微粒（EMPs）是从激活或凋亡的内皮细胞释放的载体。本研究旨在建立一种新的 EMPs 细胞计数珠粒检测方法，并探讨 EMPs 在胸痛患者中的预后价值。
我们发明并验证了细胞计数珠粒检测方法，以体外定量 EMP 水平。共招募了 80 名健康志愿者和 350 名胸痛患者，并测量了 EMPs。在随访期间记录了有明确冠状动脉疾病患者的主要不良心血管事件（MACE）。体外研究表明，EMP 水平与内皮素-1（ET-1）和细胞间黏附分子-1（ICAM-1）水平呈统计学相关。健康受试者的 EMP 水平<300.10。患者的 EMP 水平明显高于健康受试者。糖尿病、EMP 和 ET-1 水平与胸痛患者的未来心血管事件显著相关。在急性冠状动脉综合征（ACS）患者组中，EMP 水平较高的 tertile 组的事件发生率明显高于较低 tertile 组。
成功建立了一种新型 EMP 定量检测方法。体外和患者的 EMPs 与 ET-1 和 ICAM-1 水平显著相关。EMP 水平较高的患者发生 MACE 的风险更高。EMP 水平是 ACS 患者 MACE 的预测因子。
