Esmaili Mohsen, Qujeq Durdi, Yoonesi Ali Asghar, Feizi Farideh, Ranaee Mohammad
Department of Biochemistry and Biophysics, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
Cellular and Molecular Biology Research Center (CMBRC), Babol University of Medical Sciences, Babol, Iran.
Mol Biol Res Commun. 2014 Jun;3(2):141-147.
The present study aimed at investigating the beneficial effects of co-administering granulocyte colony-stimulating factor (G-CSF) and stem cell factor (SCF) in a model of chronic liver injury induced by thioacetamide (TAA). Biochemical and histopathology- cal examinations were performed on serum and liver specimens. At the end of the treatment period, the rats were anesthetized with ether, serum was collected and sections of randomly selected fixed liver specimens from each group were embedded in paraffin and processed for light microscopy by staining individual sections with hematoxylin- eosin (HE) stain. Administration of a combination of G-CSF+SCF was carried out two weeks after the TAA treatment. Livers of rats treated with TAA alone exhibited damage, which was significantly less in the group treated with the combination of SCF and G-CSF. Albumin level was 2.35 (g/dl) in the G-CSF+SCF and 1.03 in the TAA- alone group. These differences were statistically significant (P<0.05). Also, in the G- CSF+SCF and TAA group the total protein means (7.16 versus 3.57 mg/dl, respective- ly) were higher than those of the TAA-alone group, and the differences were statistically significant (P<0.05). In the G-CSF+SCF and TAA group the total bilirubin content mean (0.15 versus 0.14 mg/dl, respectively) this difference was not statistically significant (P>0.05).
本研究旨在探讨联合使用粒细胞集落刺激因子（G-CSF）和干细胞因子（SCF）对硫代乙酰胺（TAA）诱导的慢性肝损伤模型的有益作用。对血清和肝脏标本进行了生化和组织病理学检查。在治疗期结束时，用乙醚麻醉大鼠，收集血清，并将每组随机选取的固定肝脏标本切片用石蜡包埋，并用苏木精-伊红（HE）染色进行光学显微镜检查。在TAA治疗两周后给予G-CSF+SCF联合用药。单独用TAA治疗的大鼠肝脏出现损伤，而在联合使用SCF和G-CSF治疗的组中损伤明显减轻。G-CSF+SCF组白蛋白水平为2.35（g/dl），单独TAA组为1.03。这些差异具有统计学意义（P<0.05）。此外，G-CSF+SCF组和TAA组的总蛋白平均值（分别为7.16和3.57mg/dl）高于单独TAA组，差异具有统计学意义（P<0.05）。G-CSF+SCF组和TAA组的总胆红素含量平均值（分别为0.15和0.14mg/dl），该差异无统计学意义（P>0.05）。
