Zeitler P, Murphy E, Handwerger S
Life Sci. 1986 Jan 13;38(2):99-107. doi: 10.1016/0024-3205(86)90001-9.
Previous investigations from this laboratory have indicated that arachidonic acid stimulates a rapid, dose-dependent and reversible increase in hPL release which is not dependent on cyclooxygenase or lipoxygenase metabolism. To investigate further the mechanism by which arachidonic acid stimulates the release of hPL, the effect of arachidonic acid on the release of 45Ca from perifused cells prelabelled with 45CaCl was examined in an enriched cell culture population of term human syncytiotrophoblast. Arachidonic acid (10-100 microM) stimulated a dose-dependent, rapid, and reversible increase in the release of both 45Ca and hPL from the perifused placental cells. On the other hand, palmitic acid had little effect on either hPL release or 45Ca release even at concentrations as high as 100 microM. Ionophore A23187 (1-10 microM) also stimulated a dose-dependent and reversible increase in hPL release. Since arachidonic acid increases the mobilization of cellular calcium, as reflected by the increased 45calcium efflux, and since an increase in the intracellular calcium concentration appears to stimulate an increase in hPL release, these results suggest that the stimulation of hPL release by arachidonic acid may be due, at lease in part, to the effects of the fatty acid on cellular calcium mobilization.
该实验室先前的研究表明，花生四烯酸可刺激人胎盘催乳素（hPL）的释放迅速、呈剂量依赖性且可逆地增加，这一过程不依赖于环氧化酶或脂氧合酶代谢。为了进一步研究花生四烯酸刺激hPL释放的机制，在足月人合体滋养层细胞富集培养群体中，检测了花生四烯酸对预先用45CaCl标记的灌流细胞中45Ca释放的影响。花生四烯酸（10 - 100微摩尔）刺激灌流胎盘细胞中45Ca和hPL的释放呈剂量依赖性、迅速且可逆地增加。另一方面，即使在浓度高达100微摩尔时，棕榈酸对hPL释放或45Ca释放几乎没有影响。离子载体A23187（1 - 10微摩尔）也刺激hPL释放呈剂量依赖性且可逆地增加。由于花生四烯酸增加了细胞钙的动员，这由45钙外流增加反映出来，并且由于细胞内钙浓度的增加似乎刺激了hPL释放的增加，这些结果表明花生四烯酸对hPL释放的刺激作用至少部分可能归因于脂肪酸对细胞钙动员的影响。
