State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
China National Rice Research Institute, Hangzhou, Zhejiang 310000, China.
J Invertebr Pathol. 2019 May;163:8-10. doi: 10.1016/j.jip.2019.02.006. Epub 2019 Feb 23.
Cost-effective Bt resistance monitoring programmes exist to manage insect resistance to Bt crops. F2 screening is widely used in detecting rare resistant alleles. One way to establish numerous isofemale lines for F2 bioassays is acquiring many field-trapped copulated females. However, it is difficult to obtain sufficient Chilo suppressalis isofemale lines because females have low mating rates and fecundity. We developed a new method to establish C. suppressalis isofemale lines with field-collected egg masses. No significant difference in detection ability was observed in the F2 screen between the old and new methods. Moreover, the new method is economical, convenient and efficient.
具有成本效益的 Bt 抗性监测计划可用于管理昆虫对 Bt 作物的抗性。F2 筛选被广泛用于检测罕见的抗性等位基因。建立大量 F2 生物测定的同型雌系的一种方法是获取大量野外捕获的交配雌蝇。然而，由于雌蝇的交配率和繁殖力低，获得足够的二化螟同型雌系是很困难的。我们开发了一种利用野外采集的卵块建立二化螟同型雌系的新方法。新旧方法在 F2 筛选中的检测能力没有显著差异。此外，新方法经济、方便、高效。
