Shyne-Athwal S, Riccio R V, Chakraborty G, Ingoglia N A
Science. 1986 Feb 7;231(4738):603-5. doi: 10.1126/science.3080804.
Rat optic and sciatic nerves were crushed, and 10 minutes to 3 days later nerve segments between the crushed site and the cell body were removed and assayed for posttranslational protein modification by amino acid addition. Protein modification was comparable in intact optic and sciatic nerves, but in sciatic nerves increased to 1.6 times control levels 10 minutes after crushing and reached a maximum of ten times control levels by 2 hours. In optic nerves activity was decreased throughout the time course studied. The results indicate that, in a nerve which is capable of regeneration (sciatic), protein modification by the addition of amino acids increases immediately after injury, but a nerve incapable of regeneration (optic) is incapable of activating the modification reaction. These findings may be important in understanding the reasons for the lack of a regenerative response after injury to central mammalian nerves.
将大鼠的视神经和坐骨神经切断，在切断后10分钟至3天，切除切断部位与细胞体之间的神经节段，并通过氨基酸添加来检测翻译后蛋白质修饰情况。完整的视神经和坐骨神经中的蛋白质修饰情况相当，但坐骨神经在切断后10分钟时蛋白质修饰增加至对照水平的1.6倍，到2小时时达到对照水平的10倍。在整个研究的时间进程中，视神经中的活性都有所下降。结果表明，在能够再生的神经（坐骨神经）中，损伤后通过添加氨基酸进行的蛋白质修饰会立即增加，但不能再生的神经（视神经）则无法激活这种修饰反应。这些发现对于理解哺乳动物中枢神经损伤后缺乏再生反应的原因可能具有重要意义。
