Tan J, Han L, Jing Y Y, Zheng H W, Shen J X, Zhang L H, Yu L S
Department of Otolaryngology Head and Neck Surgery,People's Hospital,Peking University,Beijing,100044,China.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2019 Feb 5;33(2):171-175. doi: 10.13201/j.issn.1001-1781.2019.02.019.
To explore the role of microRNA-203 in laryngeal cancer and its underlying mechanism and clarify the relationship between microRNA-203 and LASP1. microRNA-203 expression in laryngeal cancer tissues and paracancerous tissues was detected by quantitative real time-polymerase chain reaction（qRT-PCR）. The regulatory effects of microRNA-203 on invasion and apoptosis of laryngeal cancer cells were detected by Transwell assay and flow cytometry, respectively. Dual-luciferase reporter gene assay was performed to access the binding condition of microRNA-203 and LASP1. Both mRNA and protein levels of LASP1 in laryngeal cancer cells were detected after transfection with microRNA-203 mimic or microRNA-203 inhibitor by qRT-PCR and Western blot. Rescue experiments were finally performed to detect whether microRNA-203 regulates laryngeal cancer development via targeting LASP1. microRNA-203 was lowly expressed in laryngeal cancer tissues and cell lines.Knockdown of microRNA-203 in Hep-2 cells can promote the invasiveness and inhibit apoptosis of laryngeal cancer cells. Subsequently,LASP1 was predicted to be the target gene of microRNA-203，which was further verified by dual-luciferase reporter gene assay.LASP1 expression was negatively regulated by microRNA-203. Furthermore，rescue experiments showed that microRNA-203 regulates invasion and apoptosis of laryngeal cancer cells via targeting LASP1. Low expression of microRNA-203 could promote the invasion and inhibit apoptosis of laryngeal cancer cells viainhibiting LASP1. microRNA-203 and LASP1 both play a very important role in the development of laryngeal cancer..
探讨微小RNA-203在喉癌中的作用及其潜在机制，并阐明微小RNA-203与LASP1之间的关系。采用定量实时聚合酶链反应（qRT-PCR）检测喉癌组织和癌旁组织中微小RNA-203的表达。分别通过Transwell实验和流式细胞术检测微小RNA-203对喉癌细胞侵袭和凋亡的调节作用。进行双荧光素酶报告基因实验以检测微小RNA-203与LASP1的结合情况。用微小RNA-203模拟物或微小RNA-203抑制剂转染喉癌细胞后，通过qRT-PCR和蛋白质免疫印迹法检测LASP1的mRNA和蛋白水平。最后进行挽救实验，以检测微小RNA-203是否通过靶向LASP1调节喉癌的发展。微小RNA-203在喉癌组织和细胞系中低表达。在Hep-2细胞中敲低微小RNA-203可促进喉癌细胞的侵袭并抑制其凋亡。随后，预测LASP1是微小RNA-203的靶基因，双荧光素酶报告基因实验进一步验证了这一点。LASP1的表达受到微小RNA-203的负调控。此外，挽救实验表明，微小RNA-203通过靶向LASP1调节喉癌细胞的侵袭和凋亡。微小RNA-203低表达可通过抑制LASP1促进喉癌细胞的侵袭并抑制其凋亡。微小RNA-203和LASP1在喉癌的发生发展中均发挥着非常重要的作用。
