稀释聚维酮碘对与言语相关细菌生长的影响。

The effect of diluting povidone-iodine on bacterial growth associated with speech.

作者信息

Gnanasekaran Sivashanth, Rogers Sophie, Wickremasinghe Sanj, Sandhu Sukhpal S

机构信息

Centre For Eye Research Australia, The Royal Victorian Eye and Ear Hospital, University of Melbourne, 32 Gisborne Street, East Melbourne, Victoria, 3002, Australia.

出版信息

BMC Ophthalmol. 2019 Feb 26;19(1):62. doi: 10.1186/s12886-019-1066-5.

DOI:10.1186/s12886-019-1066-5

PMID:30808316

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6390624/

Abstract

BACKGROUND

Povidone-Iodine (PI) may be diluted when used as an antiseptic prior to an intravitreal injection in an attempt to decrease patient discomfort. This study aims to investigate the effect of diluting povidone-iodine (PI) on bacterial growth from bacterial droplet dispersal associated with speech.

METHODS

Participants read a standardised script for 5 min over a blood agar plate positioned at 20 cm in a simulated position of an intravitreal injection procedure. The blood agar plates were subject to a randomised pre-application of 1% PI; 2.5% PI; 5% PI and no pre-application (control). The plates were incubated at 37 °C for 72 h and the number of Colony Forming Units (CFUs) was determined. CFUs were summarised as median and interquartile range (IQR). Wilcoxon rank sum test was used to assess pairwise comparisons of the various PI concentrations to the control group. Any trend across PI concentration was assessed using Kendall's tau rank correlation.

RESULTS

Twenty-one subjects participated. Control plates had a median growth of 25 CFUs (interquartile range [IQR]:15-40), 1% PI plates had a median growth of 30 CFUs (IQR:15-82), 2.5% PI had a median growth of 18 CFUs (IQR:10-32) and 5% PI had a median growth of 2 CFUs (IQR:0-5). There was significantly less bacterial growth with 5% PI compared to control (P < 0.001). Bacterial growth at 2.5% PI and 1% PI did not differ significantly from control. There was a statistically significant trend for decreasing colony count as PI concentration increased (P < 0.001).

CONCLUSIONS

PI concentrations less than 5% are not effective at reducing bacterial growth from bacterial droplet dispersal associated with speech. When using PI for pre-injection antisepsis, concentrations below 5% should be avoided.

摘要

背景

聚维酮碘（PI）在玻璃体内注射前用作防腐剂时，可能会被稀释，以减轻患者的不适。本研究旨在调查稀释聚维酮碘（PI）对与言语相关的细菌飞沫传播导致的细菌生长的影响。

方法

参与者在模拟玻璃体内注射程序的位置，于距离血琼脂平板20厘米处朗读标准化脚本5分钟。血琼脂平板被随机预先涂抹1% PI；2.5% PI；5% PI，以及不进行预先涂抹（对照组）。平板在37°C下孵育72小时，并确定菌落形成单位（CFU）的数量。CFU以中位数和四分位间距（IQR）进行汇总。使用Wilcoxon秩和检验评估不同PI浓度与对照组的两两比较。使用Kendall's tau秩相关评估PI浓度之间的任何趋势。

结果

21名受试者参与。对照平板的CFU中位数为25（四分位间距[IQR]：15 - 40），1% PI平板的CFU中位数为30（IQR：15 - 82），2.5% PI的CFU中位数为18（IQR：10 - 32），5% PI的CFU中位数为2（IQR：0 - 5）。与对照组相比，5% PI的细菌生长显著减少（P < 0.001）。2.5% PI和1% PI的细菌生长与对照组无显著差异。随着PI浓度增加，菌落计数有统计学显著的下降趋势（P < 0.001）。