Department of Ophthalmology, California Pacific Medical Center, San Francisco, California 94115, USA.
Retina. 2012 Mar;32(3):473-6. doi: 10.1097/IAE.0B013E31822C2958.
To provide experimental evidence to support or refute the proposition that the use of surgical face masks and/or avoidance of talking can decrease the dispersion of respiratory flora during an intravitreal injection.
Ten surgeons recited a 30-second standardized script with blood agar plates positioned 30 cm below their mouths. The plates were divided into 4 groups, with 10 plates per group. In Group 1, participants did not wear a face mask. In Group 2, participants wore a standard surgical mask. In Group 3, no mask was worn, but plates were pretreated with 5% povidone-iodine. In Group 4, no mask was worn, and participants remained silent for 30 seconds. The plates were then incubated at 37°C for 24 hours, and the number of colony-forming units (CFUs) was determined.
Mean bacterial growth were as follows: Group 1, 8.6 CFUs per subject; Group 2, 1.1 CFUs per subject; Group 3, 0.1 CFUs per subject; and Group 4, 2.4 CFUs per subject. Differences between the groups were statistically significant (P < 0.05), with the exception of Group 2 versus Group 4 (P = 0.115).
The use of a face mask and avoidance of talking each significantly decreased the dispersion of bacteria. Even without these interventions, plates pretreated with povidone-iodine demonstrated the least bacterial growth.
提供实验证据支持或反驳以下观点，即使用手术口罩和/或避免交谈可以减少眼内注射过程中呼吸菌群的扩散。
10 名外科医生用含血琼脂的培养皿对着嘴念一段 30 秒的标准化台词，培养皿放在距嘴 30 厘米处。培养皿分为 4 组，每组 10 个。第 1 组参与者不戴口罩；第 2 组戴标准手术口罩；第 3 组不戴口罩，但培养皿预先用 5%聚维酮碘处理；第 4 组不戴口罩且参与者保持 30 秒沉默。然后将培养皿在 37°C 下孵育 24 小时，确定菌落形成单位（CFU）数量。
平均细菌生长情况如下：第 1 组，每个参与者 8.6 CFU；第 2 组，每个参与者 1.1 CFU；第 3 组，每个参与者 0.1 CFU；第 4 组，每个参与者 2.4 CFU。各组之间的差异具有统计学意义（P<0.05），除第 2 组与第 4 组（P=0.115）外。
使用口罩和避免交谈均可显著减少细菌的扩散。即使不进行这些干预，用聚维酮碘预处理的培养皿显示出的细菌生长最少。
