Morii S, Ngai A C, Ko K R, Winn H R
Am J Physiol. 1986 Feb;250(2 Pt 2):H304-12. doi: 10.1152/ajpheart.1986.250.2.H304.
We analyzed the retroglenoid venous outflow (VOF) technique in the rat to document the validity of this method of measuring cerebral blood flow (CBF). Stereotypic changes in CBF were obtained with VOF during hypercarbia and hypotension. O2 content of retroglenoid venous blood did not differ significantly from O2 content of blood obtained from the sagittal sinus, suggesting minimal extracerebral contamination of the retroglenoid venous blood. This lack of extracerebral contamination was further analyzed using a double tracer technique (125I-labeled serum albumin, 22Na) that quantitated minimal extracerebral contamination in the retroglenoid vein. CBF measurements were made simultaneously using microsphere and VOF methods, and excellent correlation was found between the two techniques over a wide range of CBF during normoxia, hypoxia, and normoxic hypocarbia and hypercarbia. However, a decrease in the ratio of VOF to microsphere CBF was observed during severe normoxic hypotension (mean arterial pressure = 41 +/- 4 mmHg). VOF represented 18% of total CBF as measured by microsphere method. This study indicates that the retroglenoid outflow technique in rats is a valid method of measuring CBF.
我们分析了大鼠的关节盂后静脉流出量（VOF）技术，以证明这种测量脑血流量（CBF）方法的有效性。在高碳酸血症和低血压期间，通过VOF获得了CBF的刻板变化。关节盂后静脉血的氧含量与从矢状窦获得的血液的氧含量没有显著差异，这表明关节盂后静脉血的脑外污染最小。使用双示踪技术（125I标记的血清白蛋白，22Na）进一步分析了这种脑外污染的缺乏，该技术定量了关节盂后静脉中最小的脑外污染。同时使用微球法和VOF方法进行CBF测量，发现在常氧、低氧以及常氧性低碳酸血症和高碳酸血症期间，在很宽的CBF范围内，这两种技术之间具有良好的相关性。然而，在严重的常氧性低血压（平均动脉压 = 41 +/- 4 mmHg）期间，观察到VOF与微球法测量的CBF之比降低。VOF占微球法测量的总CBF的18%。这项研究表明，大鼠的关节盂后流出技术是一种有效的测量CBF的方法。
