Brucato M, Sundlof S F, Bell J U, Edds G T
Am J Vet Res. 1986 Jan;47(1):179-83.
Effects of a single IM injection of selenium-vitamin E (Se-E; 5 mg of Se + 68 IU of alpha-tocopherol/60 kg of body weight) as a pretreatment 14 days before an oral dose of aflatoxin B1 (1.0 mg/kg) were studied in 24 dairy calves. Treatment groups were designated as follows: group 1 = no Se-E or aflatoxin B1 (control); group 2 = Se-E supplementation only; group 3 = aflatoxin B1 dose only; and group 4 = Se-E supplementation before aflatoxin B1 dose. Clinical signs of toxicosis in aflatoxin B1-treated calves included anorexia, ataxia, rough haircoats, increased respiration rates, dyspnea, dehydration, and nasal discharge. Packed-cell volume, RBC, WBC, and hemoglobin were increased in aflatoxin-treated calves. Significant increases in serum aspartate aminotransferase (P less than 0.05) and gamma-glutamyl-transferase (P less than 0.001) activities and prothrombin times (P less than 0.001) were observed in aflatoxin-treated calves, indicating that there was hepatic involvement. Although aflatoxin exposure caused a significant decrease in body weight (P less than 0.01) and feed intake (P less than 0.001) in treatment groups 3 and 4, Se was demonstrated to interact significantly (P less than 0.001) with aflatoxin B1 for feed intake, causing an improved feed intake in treatment group 4 calves.
在24头犊牛中研究了在口服黄曲霉毒素B1（1.0毫克/千克）前14天单次肌肉注射硒 - 维生素E（Se - E；5毫克硒 + 68国际单位α - 生育酚/60千克体重）作为预处理的效果。治疗组划分如下：第1组 = 不注射Se - E或不给予黄曲霉毒素B1（对照组）；第2组 = 仅补充Se - E；第3组 = 仅给予黄曲霉毒素B1剂量；第4组 = 在给予黄曲霉毒素B1剂量前补充Se - E。接受黄曲霉毒素B1处理的犊牛中毒的临床症状包括厌食、共济失调、被毛粗糙、呼吸频率增加、呼吸困难、脱水和鼻分泌物。接受黄曲霉毒素处理的犊牛的红细胞压积、红细胞、白细胞和血红蛋白增加。在接受黄曲霉毒素处理的犊牛中观察到血清天冬氨酸转氨酶（P < 0.05）和γ - 谷氨酰转移酶（P < 0.001）活性以及凝血酶原时间（P < 0.001）显著增加，表明有肝脏受累。尽管黄曲霉毒素暴露导致第3组和第4组治疗组的体重（P < 0.01）和采食量（P < 0.001）显著下降，但硒被证明与黄曲霉毒素B1在采食量方面有显著相互作用（P < 0.001），使第4组犊牛的采食量有所改善。
