Ye Zhongju, Liu Hua, Wang Fuyan, Wang Xin, Wei Lin, Xiao Lehui
State Key Laboratory of Medicinal Chemical Biology , Tianjin Key Laboratory of Biosensing and Molecular Recognition , College of Chemistry , Nankai University , Tianjin , 300071 , China . Email:
Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research , Key Laboratory of Phytochemical R&D of Hunan Province , College of Chemistry and Chemical Engineering , Hunan Normal University , Changsha , 410082 , China.
Chem Sci. 2018 Nov 12;10(5):1351-1359. doi: 10.1039/c8sc04033h. eCollection 2019 Feb 7.
It has been demonstrated that rod-shaped particles can achieve a high translocation efficiency for gene and drug delivery in biological samples. Previous theoretical calculations also confirmed that rod-shaped particles display higher diffusivity than their spherical counterparts in biological porous media. Understanding the diffusion dynamics of biological and non-biological rod-shaped particles in biological solutions as well as close to the lipid membrane is therefore fundamentally significant for the rational design of efficient cargos. With dark-field optical microscopy, the translational and three-dimensional (3D) orientational diffusion dynamics of individual rod-shaped particles (, and upconversion microrods, UCMRs) in phosphate buffered saline (PBS) and on the lipid membrane are tracked at the single-particle level. In the buffer solution, faster rotation of in the direction was observed even though its dynamics in the - plane is comparable with that of UCMRs. Interestingly, on the lipid membrane, distinct from the confined motion of UCMRs, anomalous rocking diffusion was observed, which might facilitate the subsequent survey of stronger association sites on the two-dimensional (2D) surface. These results would afford deep insight into the better understanding of the translocation mechanism by using rod-shaped particles as a delivery cargo in biological samples.
已证明棒状颗粒在生物样品中进行基因和药物递送时可实现高转运效率。先前的理论计算也证实，在生物多孔介质中，棒状颗粒比球形颗粒表现出更高的扩散率。因此，了解生物和非生物棒状颗粒在生物溶液中以及靠近脂质膜时的扩散动力学，对于合理设计高效载体至关重要。利用暗场光学显微镜，在单颗粒水平上追踪了单个棒状颗粒（、和上转换微棒，UCMRs）在磷酸盐缓冲盐水（PBS）中以及脂质膜上的平移和三维（3D）取向扩散动力学。在缓冲溶液中，尽管其在平面内的动力学与UCMRs相当，但观察到在方向上旋转更快。有趣的是，在脂质膜上，与UCMRs的受限运动不同，观察到了异常摇摆扩散，这可能有助于随后对二维（2D）表面上更强结合位点的探测。这些结果将为更好地理解在生物样品中使用棒状颗粒作为递送载体的转运机制提供深入见解。
