Department of Soil Science, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Microb Ecol. 1987 Jul;14(1):1-14. doi: 10.1007/BF02011566.
Phase, darkfield, and computer-enhanced microscopy were used to observe the surface microenvironment of flow cells during bacterial colonization. Microbial behavior was consistent with the assumptions used previously to derive surface colonization kinetics and to calculate surface growth and attachment rates from cell number and distribution. Surface microcolonies consisted of closely packed cells. Each colony contained 2(n) cells, where n is the number of cell divisions following attachment. Initially, cells were freely motile while attached, performing circular looping movements within the plane of the solid-liquid interface. Subsequently, cells attached apically, maintained a fixed position on the surface, and rotated. This type of attachment was reversible and did not necessarily lead to the formation of microcolonies. Cells became irreversibly attached by progressing from apical to longitudinal attachment. Longitudinally attached cells increased in length, then divided, separated, moved apart laterally, and slid next to one another. This resulted in tight cell packing and permitted simultaneous growth and adherence. After approximately 4 generations, individual cells emigrated from developing microcolonies to recolonize the surface at new locations. Surface colonization byPseudomonas fluorescens can thus be subdivided into the following sequential colonization phases: motile attachment phase, reversible attachment phase, irreversible attachment phase, growth phase, and recolonization phase.
采用相衬、暗场和计算机增强显微镜观察细菌定殖过程中流动池的表面微环境。微生物的行为与之前用于推导表面定殖动力学和从细胞数量和分布计算表面生长和附着速率的假设一致。表面微菌落由紧密堆积的细胞组成。每个菌落包含 2(n)个细胞，其中 n 是附着后细胞分裂的次数。最初，细胞附着时可以自由运动，在固液界面的平面内进行圆形环行运动。随后，细胞顶端附着，在表面保持固定位置并旋转。这种附着类型是可逆的，不一定会导致微菌落的形成。细胞通过从顶端附着到纵向附着而不可逆地附着。纵向附着的细胞变长，然后分裂、分离、侧向移动并彼此相邻滑动。这导致细胞紧密包装，并允许同时生长和附着。大约 4 代后，单个细胞从发育中的微菌落中迁出，在新位置重新殖民表面。因此，荧光假单胞菌的表面定殖可以细分为以下连续的定殖阶段：游动附着阶段、可逆附着阶段、不可逆附着阶段、生长阶段和再殖民阶段。
