Faculty of Life and Environmental Sciences, University of Tsukuba, Tsukuba City 305-8572, Japan.
Master's/Doctoral Program in Life Science Innovation, School of Integrative and Global Majors (SIGMA), University of Tsukuba, Tsukuba City 305-8572, Japan.
Biomed Res Int. 2019 Jan 28;2019:3104057. doi: 10.1155/2019/3104057. eCollection 2019.
4W-1b (AL4W-1b) is a newly discovered microalgal strain with unique features. In the present study, we investigated the effects of ethanol extracts of AL4W-1b on lipopolysaccharide- (LPS-) induced inflammatory responses in RAW264 murine macrophage cells. Pretreatment of RAW264 cells with the AL4W-1b extract significantly reduced the production of LPS-induced nitric oxide (NO) and the expression of proinflammatory cytokine genes, including tumor necrosis factor , interleukin- (IL-) 1, and IL-6. Treatment with the AL4W-1b extract also decreased the production of IL-1 and IL-6. These results suggest that AL4W-1b might have anti-inflammatory effects in RAW264 cells. The NF-B inhibitor, BAY 11-7082, synergistically prevented LPS-induced NO production after pretreatment with the AL4W-1b extract. Thus, the AL4W-1b extract may affect not only the NF-B pathway but also other inflammatory pathways. To the best of our knowledge, this is the first study to report the anti-inflammatory effects of AL4W-1b extract and its mechanism of action in LPS-stimulated murine macrophage cells.
4W-1b（AL4W-1b）是一种新发现的具有独特特征的微藻菌株。在本研究中，我们研究了 AL4W-1b 的乙醇提取物对脂多糖-（LPS-）诱导的 RAW264 鼠巨噬细胞炎症反应的影响。AL4W-1b 提取物预处理 RAW264 细胞显著降低了 LPS 诱导的一氧化氮（NO）的产生和促炎细胞因子基因的表达，包括肿瘤坏死因子-α、白细胞介素-（IL-）1 和 IL-6。AL4W-1b 提取物的处理还降低了 IL-1 和 IL-6 的产生。这些结果表明，AL4W-1b 可能对 RAW264 细胞具有抗炎作用。NF-B 抑制剂 BAY 11-7082 与 AL4W-1b 提取物预处理协同防止 LPS 诱导的 NO 产生。因此，AL4W-1b 提取物可能不仅影响 NF-B 途径，还影响其他炎症途径。据我们所知，这是第一项报道 AL4W-1b 提取物的抗炎作用及其在 LPS 刺激的鼠巨噬细胞中的作用机制的研究。
