Berger S J, Sudar D C, Berger N A
Biochem Biophys Res Commun. 1986 Jan 14;134(1):227-32. doi: 10.1016/0006-291x(86)90551-6.
In this communication we show that activation of poly(ADP-ribose) polymerase by DNA damage can produce drastic alterations in carbohydrate metabolism. We examined alterations in NAD+, NADP+, ATP and glucose-6-phosphate in L1210 murine leukemia cells, following exposure to different concentrations of N-methyl-N'-nitro-N-nitrosoguanidine. Treatment of cells with 20 micrograms/ml MNNG produced rapid depletion of NAD+ and ATP. The G-6-P pool showed a biphasic change: first the pool size decreased, then increased to a level greater than that present in control cells. Nicotinamide treatment prevented the total depletion of NAD+ and this in turn helped preserve the ATP pools and prevented the biphasic alteration in G-6-P pool sizes.
在本通讯中，我们表明DNA损伤激活聚（ADP - 核糖）聚合酶可导致碳水化合物代谢发生剧烈改变。我们检测了L1210小鼠白血病细胞在暴露于不同浓度的N - 甲基 - N'-硝基 - N - 亚硝基胍后，NAD⁺、NADP⁺、ATP和葡萄糖 - 6 - 磷酸的变化。用20微克/毫升的MNNG处理细胞会导致NAD⁺和ATP迅速耗竭。葡萄糖 - 6 - 磷酸池呈现双相变化：首先池大小减小，然后增加到高于对照细胞中的水平。烟酰胺处理可防止NAD⁺完全耗竭，这反过来有助于维持ATP池，并防止葡萄糖 - 6 - 磷酸池大小的双相改变。
