Dallinga-Thie G M, van Tol A, van't Hooft F M, Groot P H
Biochim Biophys Acta. 1986 Mar 21;876(1):108-15. doi: 10.1016/0005-2760(86)90323-1.
The distribution of apolipoproteins A-I and A-IV among lymph lipoprotein fractions was studied after separation by molecular sieve chromatography, avoiding any ultracentrifugation. Lymph was obtained from rats infused either with a glucose solution or with a triacylglycerol emulsion. Relative to glucose infusion, triacylglycerol infusion caused a 20-fold increase in the output of triacylglycerol, coupled with a 4-fold increase in output of apolipoprotein A-IV. The output of apolipoprotein A-I was only elevated 2-fold. Chromatography on 6% agarose showed that lymph apolipoproteins A-I and A-IV are present on triacylglycerol-rich particles and on particles of the size of HDL. In addition, apolipoprotein A-IV is also present as 'free' apolipoprotein A-IV. The increase in apolipoprotein A-I output is caused by a higher output of A-I associated with large chylomicrons only, while the increase in apolipoprotein A-IV output is reflected by an increased output in all lymph lipoprotein fractions, including lymph HDL and 'free' apolipoprotein A-IV. The increased level of 'free' A-IV, seen in fatty lymph, may contribute to, and at least partly explain, the high concentrations of 'free' apolipoprotein A-IV present in serum obtained from fed animals.
在不进行任何超速离心的情况下，通过分子筛色谱分离后，研究了载脂蛋白A-I和A-IV在淋巴脂蛋白组分中的分布。淋巴液取自输注葡萄糖溶液或三酰甘油乳剂的大鼠。相对于输注葡萄糖，输注三酰甘油使三酰甘油的输出量增加了20倍，同时载脂蛋白A-IV的输出量增加了4倍。载脂蛋白A-I的输出量仅升高了2倍。在6%琼脂糖上进行色谱分析表明，淋巴载脂蛋白A-I和A-IV存在于富含三酰甘油的颗粒以及高密度脂蛋白大小的颗粒上。此外，载脂蛋白A-IV也以“游离”载脂蛋白A-IV的形式存在。载脂蛋白A-I输出量的增加仅由与大乳糜微粒相关的A-I输出量增加引起，而载脂蛋白A-IV输出量的增加则反映在所有淋巴脂蛋白组分中，包括淋巴高密度脂蛋白和“游离”载脂蛋白A-IV的输出量增加。在富含脂肪的淋巴中观察到的“游离”A-IV水平升高，可能有助于并至少部分解释从喂食动物获得的血清中存在的高浓度“游离”载脂蛋白A-IV。
