Jungi T W, Spycher M O, Nydegger U E, Barandun S
Blood. 1986 Mar;67(3):629-36.
The association of platelets with leukocytes was investigated, using gel-filtered platelets stimulated with thrombin and then fixed with formaldehyde. Evidence is presented that stimulation of gel-filtered platelets with low concentrations of thrombin (0.01 to 0.1 U/mL) induces the expression of surface determinants interacting strongly with monocytes and polymorphonuclear leukocytes (PMNs) but only weakly with lymphocytes. Both monocyte-platelet binding and PMN-platelet binding occurred at 37 degrees C and more intensively at 0 degrees C; it was Ca2+-dependent and was unaffected by the addition of sodium azide. The binding also occurred with the monocytoid cell lines HL 60 and U 937 in exponential growth and was much less two days after induction of terminal differentiation by phorbol myristate acetate (PMA). No other tested cell lines (B cells, T cells, and myeloid cells) bound thrombin-stimulated platelets. Monocyte-derived macrophages kept in culture for one week also exhibited reduced binding of thrombin-stimulated platelets. IgG and fibronectin could be ruled out as ligands that mediate binding.
利用经凝胶过滤的血小板，用凝血酶刺激后再用甲醛固定，对血小板与白细胞的关联进行了研究。有证据表明，用低浓度凝血酶（0.01至0.1 U/mL）刺激经凝胶过滤的血小板会诱导表面决定簇的表达，这些决定簇与单核细胞和多形核白细胞（PMN）强烈相互作用，但与淋巴细胞的相互作用较弱。单核细胞与血小板的结合以及PMN与血小板的结合均在37℃时发生，在0℃时更为强烈；它依赖于Ca2+，且不受叠氮化钠添加的影响。这种结合在指数生长期的单核细胞样细胞系HL 60和U 937中也会发生，在用佛波酯（PMA）诱导终末分化两天后结合则少得多。没有其他测试的细胞系（B细胞、T细胞和髓细胞）能与凝血酶刺激的血小板结合。在培养一周的单核细胞衍生巨噬细胞中，凝血酶刺激的血小板的结合也减少。可以排除IgG和纤连蛋白作为介导结合的配体。
