Department of Genetics, Microbiology and Statistics, Universitat de Barcelona, 08028, Barcelona, Catalonia, Spain.
Institute of Environmental Assessment and Water Research (IDAEA, CSIC), 08034, Barcelona, Catalonia, Spain.
Int Microbiol. 2018 Dec;21(4):231-235. doi: 10.1007/s10123-018-0020-3. Epub 2018 Jul 16.
Microbial mats are prokaryotic communities that provide model systems to analyze microbial diversity and ecophysiological interactions. Sulfate-reducing bacteria (SRB) play a key role in sulfur and nutrient recycling in these ecosystems. In this work, specific primers for 16S rRNA encoding gene, previously described, were used to study the diversity of SRB in microbial mats of the Ebro Delta. We confirm that this method is reliable to identify the diversity of SRB in these ecosystems. However, some mismatches in obtained sequences had been observed in our system and must be taken under consideration. Various genera of SRB in Ebro Delta microbial mats were identified, such as Desulfonema, Desulfatitalea, Desulfosalsimonas, Desulfoccocus, and Desulfovibrio. The diversity observed in our samples is very similar to previously reported in other microbial mats communities.
微生物席是原核生物群落，为分析微生物多样性和生态生理相互作用提供了模型系统。硫酸盐还原菌 (SRB) 在这些生态系统的硫和养分循环中发挥着关键作用。在这项工作中，使用了先前描述的编码基因 16S rRNA 的特定引物来研究埃布罗三角洲微生物席中 SRB 的多样性。我们证实该方法可用于可靠地识别这些生态系统中 SRB 的多样性。然而，在我们的系统中观察到一些获得的序列存在错配，这必须加以考虑。在埃布罗三角洲微生物席中鉴定出了各种硫酸盐还原菌属，如脱硫单胞菌属、脱硫芽胞杆菌属、脱硫盐单胞菌属、脱硫球菌属和脱硫弧菌属。我们的样本中观察到的多样性与之前在其他微生物席群落中报道的非常相似。
