National Wine and Grape Industry Centre , Wagga Wagga , NSW 2678 , Australia.
School of Agricultural and Wine Sciences , Charles Sturt University , Locked Bag 588 , Wagga Wagga , NSW 2678 , Australia.
J Agric Food Chem. 2019 Mar 27;67(12):3502-3510. doi: 10.1021/acs.jafc.8b07021. Epub 2019 Mar 13.
This work outlines a rapid novel methodology for the direct quantitation of 12 volatile aldehyde compounds related to oxidative off-flavors in wine, by measuring the combined free and hydrogen-sulfite-bound forms of each aldehyde compound, consisting of four general aldehydes, four Strecker aldehydes, and four (E)-2-alkenals. The methodology requires minimal preparation of wine samples: the addition of internal standards and 6 g/L sulfur dioxide, and filtration prior to quantitation by liquid chromatography-triple quadrupole-mass spectrometery. Overall, the limit of detection, limit of quantification, accuracy (recovery, 97-114%), and precision (repeatability and reproducibility, RSD ≤ 10%) were satisfactory to enable routine measurement of the 12 aldehyde compounds in wine. The methodology was applied to 20 commercial white and red wines from various varieties and vintages. A general trend of higher concentrations of the aldehyde compounds in white wines compared to red wines was observed.
本工作概述了一种快速新颖的方法，用于通过测量每种醛化合物的游离和亚硫酸氢盐结合形式，直接定量葡萄酒中与氧化异味相关的 12 种挥发性醛化合物，这些醛化合物包括四种一般醛、四种斯特雷克醛和四种（E）-2-烯醛。该方法对葡萄酒样品的制备要求很低：只需添加内标和 6 g/L 二氧化硫，然后在通过液相色谱-三重四极杆质谱定量之前进行过滤。总的来说，检测限、定量限、准确度（回收率，97-114%）和精密度（重复性和再现性，RSD≤10%）令人满意，可用于常规测量葡萄酒中的 12 种醛化合物。该方法应用于来自不同品种和年份的 20 种商业白葡萄酒和红葡萄酒。观察到与红葡萄酒相比，白葡萄酒中醛化合物的浓度普遍较高。
