Fleischmann W R, Fleischmann C M, Gindhart T D
Cancer Res. 1986 Apr;46(4 Pt 1):1722-6.
Previous studies have evaluated the effects of hyperthermia on the antiproliferative activity of interferon. The activities of all three types of interferon have been shown to be synergistically enhanced by hyperthermic conditions. Further, the antiproliferative activity of interferon has been shown to be synergistically enhanced by combinations of gamma-plus alpha- or beta-interferon. The question remained whether combining these two methods of enhancing interferon activity would lead to an even higher level of enhancement of antiproliferative activity or to an antagonism of their separate effects. To address this question, mouse B-16 melanoma cells were cloned at 37.3 degrees C and at 39.4 degrees C in the presence of various combinations of murine alpha/beta- and gamma-interferon. Potentiation of interferon's antiproliferative activity by combination interferon treatment was found to occur at both temperatures. Moreover, the level of potentiation was synergistically enhanced by hyperthermic conditions. The results suggest that a combined treatment regimen of hyperthermia and combination interferon therapy (gamma- plus alpha- or beta-interferon) may provide a highly potent antitumor effect.
以往的研究评估了热疗对干扰素抗增殖活性的影响。已表明，在热疗条件下，所有三种类型干扰素的活性均会协同增强。此外，γ干扰素与α或β干扰素联合使用时，干扰素的抗增殖活性也会协同增强。问题在于，将这两种增强干扰素活性的方法结合起来，是否会导致抗增殖活性得到更高程度的增强，或者两种方法的单独效应是否会相互拮抗。为解决这个问题，在37.3℃和39.4℃下，于存在各种组合的小鼠α/β干扰素和γ干扰素的情况下，对小鼠B - 16黑色素瘤细胞进行克隆。结果发现，在这两个温度下，联合干扰素治疗均能增强干扰素的抗增殖活性。此外，热疗条件能协同增强这种增强水平。结果表明，热疗与联合干扰素治疗（γ干扰素与α或β干扰素联合）的联合治疗方案可能会产生高效的抗肿瘤效果。
