Tanum G
Exp Hematol. 1986 Mar;14(3):202-6.
The megakaryocytopoiesis in rats was studied following a single dose of thio-TEPA to determine the mechanisms for the thrombocytopenia and subsequent recovery. The blood platelet number, platelet production (measured by 35S incorporation into platelets), mean platelet volume, and the number and DNA content of bone marrow megakaryocytes (MK) were observed. The blood platelet counts, platelet production, and total MK number decreased to low values following the administration of thio-TEPA, and stayed low until regeneration started around day 10. The mean platelet volume increased from the normal 6.6 fl to 7.8 fl during the early regeneration days 8-14. The MK were divided into four ploidy classes: 2N-4N, 8N, 16N, and 32N-64N. The number of MK within all ploidy classes decreased to nearly zero within four days after the injection of thio-TEPA. The regeneration started around day 10 with increasing numbers of 2N-4N and 8N MK, while the number of 16N and 32N-64N MK increased more than four days later. It is concluded that decreased or blocked influx of progenitor cells into the MK compartment is the main reason for thrombocytopenia after exposure to thio-TEPA.
研究了大鼠单次注射硫替派后巨核细胞生成情况，以确定血小板减少及其后续恢复的机制。观察了血小板数量、血小板生成（通过35S掺入血小板来测定）、平均血小板体积以及骨髓巨核细胞（MK）的数量和DNA含量。注射硫替派后，血小板计数、血小板生成及MK总数降至低值，并持续保持低值直至第10天左右开始再生。在再生早期的第8 - 14天，平均血小板体积从正常的6.6飞升增至7.8飞升。MK分为四个倍体类别：2N - 4N、8N、16N和32N - 64N。注射硫替派后四天内，所有倍体类别的MK数量均降至几乎为零。再生在第10天左右开始，2N - 4N和8N的MK数量增加，而16N和32N - 64N的MK数量在四天后增加得更多。得出结论，祖细胞流入MK区室减少或受阻是接触硫替派后血小板减少的主要原因。
