Mitchell C G, O'Neil S, Reeves H C, Weitzman P D
FEBS Lett. 1986 Feb 17;196(2):211-4. doi: 10.1016/0014-5793(86)80248-4.
Fast protein liquid chromatography (FPLC) has been shown to be a rapid and effective method of separating isoenzymes of citrate synthase and isocitrate dehydrogenase in extracts of Pseudomonas aeruginosa and Acinetobacter calcoaceticus. The advantages of FPLC over conventional methods of fractionation are discussed and it is suggested that this may be a valuable and more general technique for isoenzyme resolution.
快速蛋白质液相色谱法（FPLC）已被证明是一种快速有效的方法，可用于分离铜绿假单胞菌和醋酸钙不动杆菌提取物中的柠檬酸合酶和异柠檬酸脱氢酶的同工酶。文中讨论了FPLC相对于传统分级分离方法的优势，并指出这可能是一种用于同工酶分离的有价值且更通用的技术。
