促黄体生成激素释放激素刺激成年大鼠格拉夫卵泡颗粒细胞的纤溶酶原激活物，并抑制其类固醇生成。

LHRH stimulates plasminogen activator and inhibits steroid production by granulosa cells of adult rat Graafian follicles.

作者信息

Wang C, Leung A

出版信息

Mol Cell Endocrinol. 1986 Jan;44(1):61-8. doi: 10.1016/0303-7207(86)90106-1.

Abstract

We studied the effects of LHRH and its analogs on plasminogen activator production and progesterone and estradiol secretion by granulosa cells isolated from adult rat Graafian follicles. LHRH and its agonist ([des- -Gly10,D-Trp6,Pro9-NHEt]LHRH) stimulated small but significant increases in plasminogen activator production. This stimulatory action of LHRH was blocked by the addition of the specific antagonist ([D- pGlu1,D-Phe2,D-Trp3,6]LHRH). In contrast, LHRH treatment had no significant effect on basal steroid production by granulosa cells of Graafian follicles cultured for 2-6 days. However, LHRH decreased the FSH-stimulated steroidogenesis. This inhibitory effect of LHRH on steroidogenesis was abolished by concomitant addition of the LHRH antagonist. The results show that LHRH has a stimulatory effect on plasminogen activator production and a suppressive action on steroidogenesis in granulosa cells isolated from Graafian follicles of adult rats.

摘要

我们研究了促黄体生成激素释放激素（LHRH）及其类似物对从成年大鼠格拉夫卵泡分离的颗粒细胞中纤溶酶原激活物生成以及孕酮和雌二醇分泌的影响。LHRH及其激动剂（[去甘氨酸10，D-色氨酸6，脯氨酸9-乙基酰胺]LHRH）刺激纤溶酶原激活物生成出现小幅度但显著的增加。LHRH的这种刺激作用可通过添加特异性拮抗剂（[D-焦谷氨酸1，D-苯丙氨酸2，D-色氨酸3,6]LHRH）而被阻断。相反，LHRH处理对培养2 - 6天的格拉夫卵泡颗粒细胞的基础类固醇生成没有显著影响。然而，LHRH降低了促卵泡生成素刺激的类固醇生成。LHRH拮抗剂的同时添加消除了LHRH对类固醇生成的这种抑制作用。结果表明，LHRH对从成年大鼠格拉夫卵泡分离的颗粒细胞中的纤溶酶原激活物生成具有刺激作用，而对类固醇生成具有抑制作用。