Romagnani S, Maggi E, Parronchi P, Macchia D, Del Prete G F, Rossi-Ferrini P L, Ricci M, Moretta L
Int J Cancer. 1986 Mar 15;37(3):343-9. doi: 10.1002/ijc.2910370304.
T-lymphocyte populations isolated from spleens of untreated patients with Hodgkin's disease (HD) were grown by combining limiting dilution techniques and an assay system that allows clonal proliferation of virtually all human T cells. Under these conditions, high proportions of splenic T lymphocytes (50-80%) underwent clonal expansion, so that the set of clones obtained could be considered representative of the starting T-cell population. A total number of 229 clones from 6 HD spleens (3 uninvolved and 3 histologically involved by the disease) and 133 clones from 3 control spleens (obtained from otherwise healthy individuals, who underwent post-traumatic splenectomy) were examined for surface markers and tested in functional assays. One hundred and seventy-five clones from HD spleens and 75 clones from control spleens expressed the "helper/inducer" (T3+ T4+ T8-)phenotype, whereas as 54 clones from HD spleens and 58 control clones expressed the "cytotoxic/suppressor" (T3+T4-T8+) phenotype. As assessed by a non-specific lectin-dependent lytic assay, the proportion of HD clones displaying cytolytic activity was higher than that of cytolytic clones derived from control spleens. The majority of T4+ clones obtained from HD spleens (either uninvolved or histologically involved by the disease) were cytolytic, whereas only a small proportion (less than 10%) of T4+ clones derived from normal peripheral blood or spleens displayed cytolytic activity. The cytolytic potential of T4+ clones obtained from HD spleens did not reflect the activity of natural killer (NK) cells, since a minority of these clones exerted NK activity on K562 target cells. In addition, most of the T4+ cytolytic clones derived from HD spleens produced particularly high amounts of interleukin-2 (IL-2). These data indicate that T lymphocytes which concentrate in spleens of patients with HD consist at least in part of an infrequent T4+ cell subset co-expressing cytolytic activity and production of IL-2. These cells may reflect a cytotoxic reaction against unknown antigens associated with self class-II histocompatibility antigens.
从未经治疗的霍奇金病（HD）患者脾脏中分离出的T淋巴细胞群体，通过结合有限稀释技术和一种能使几乎所有人类T细胞进行克隆增殖的检测系统进行培养。在这些条件下，高比例的脾T淋巴细胞（50 - 80%）经历了克隆扩增，因此获得的克隆集可被视为起始T细胞群体的代表。对来自6个HD脾脏（3个未受累和3个组织学上受累）的总共229个克隆以及来自3个对照脾脏（从因创伤后行脾切除术的其他健康个体获得）的133个克隆进行了表面标志物检测，并在功能检测中进行了测试。来自HD脾脏的175个克隆和来自对照脾脏的75个克隆表达“辅助/诱导”（T3 + T4 + T8 +）表型，而来自HD脾脏的54个克隆和58个对照克隆表达“细胞毒性/抑制”（T3 + T4 - T8 +）表型。通过非特异性凝集素依赖性溶细胞检测评估，显示溶细胞活性的HD克隆比例高于来自对照脾脏的溶细胞克隆比例。从HD脾脏获得的大多数T4 +克隆（无论未受累还是组织学上受累）具有溶细胞活性，而从正常外周血或脾脏获得的T4 +克隆中只有一小部分（不到10%）具有溶细胞活性。从HD脾脏获得的T4 +克隆的溶细胞潜力并不反映自然杀伤（NK）细胞的活性，因为这些克隆中少数对K562靶细胞发挥NK活性。此外，大多数来自HD脾脏的T4 +溶细胞克隆产生特别大量的白细胞介素-2（IL - 2）。这些数据表明，聚集在HD患者脾脏中的T淋巴细胞至少部分由一个罕见的T4 +细胞亚群组成，该亚群共表达溶细胞活性和IL - 2的产生。这些细胞可能反映了针对与自身II类组织相容性抗原相关的未知抗原的细胞毒性反应。
