Lenich A C, Goodman S I
J Biol Chem. 1986 Mar 25;261(9):4090-6.
Glutaryl-CoA dehydrogenase, a multifunctional enzyme responsible for dehydrogenation and decarboxylation of glutaryl-CoA to crotonyl-CoA, has been purified 1,680-fold from porcine liver mitochondria. The purified porcine enzyme has a subunit molecular weight of 47,800 and a native molecular weight of 190,500. Porcine glutaryl-CoA dehydrogenase catalyzed the conversion of [1,5-14C]glutaryl-CoA to [14C] crotonyl-CoA and 14CO2 in a 1:1:1 ratio. The porcine enzyme has Km values for electron transfer flavoprotein and glutaryl-CoA of 1.1 and 3.3 microM, respectively, and turnover numbers of 860 mol of electron transfer flavoprotein/min/mol of glutaryl-CoA dehydrogenase and 327 mol of glutaryl-CoA/min/mol of glutaryl-CoA dehydrogenase. Human glutaryl-CoA dehydrogenase has been purified 1,278-fold from human liver mitochondria. The purified human enzyme has a subunit molecular weight of 58,800 and a native molecular weight of 256,000. Human glutaryl-CoA dehydrogenase showed a reaction of only partial identity when compared to porcine glutaryl-CoA dehydrogenase by Ouchterlony double immunodiffusion analysis using antiserum raised against and monospecific for porcine glutaryl-CoA dehydrogenase.
戊二酰辅酶A脱氢酶是一种多功能酶，负责将戊二酰辅酶A脱氢并脱羧生成巴豆酰辅酶A，已从猪肝线粒体中纯化了1680倍。纯化后的猪源酶亚基分子量为47,800，天然分子量为190,500。猪源戊二酰辅酶A脱氢酶催化[1,5-14C]戊二酰辅酶A以1:1:1的比例转化为[14C]巴豆酰辅酶A和14CO2。该猪源酶对电子传递黄素蛋白和戊二酰辅酶A的Km值分别为1.1和3.3微摩尔，转换数分别为860摩尔电子传递黄素蛋白/分钟/摩尔戊二酰辅酶A脱氢酶和327摩尔戊二酰辅酶A/分钟/摩尔戊二酰辅酶A脱氢酶。人源戊二酰辅酶A脱氢酶已从人肝线粒体中纯化了1278倍。纯化后的人源酶亚基分子量为58,800，天然分子量为256,000。使用针对猪源戊二酰辅酶A脱氢酶产生的抗血清进行免疫双扩散分析时，与人源戊二酰辅酶A脱氢酶相比，猪源戊二酰辅酶A脱氢酶仅显示出部分同一性反应。
