Holter W, Grunow R, Stockinger H, Knapp W
J Immunol. 1986 Mar 15;136(6):2171-5.
The present report describes the inducibility of IL 2 receptors on human peripheral blood monocytes. Although freshly isolated monocytes are IL 2 receptor negative, approximately one-third of the cells react with the anti-Tac antibody within 18 hr of culture. IFN-gamma is found to double both the number of positive cells and the number of binding sites, whereas IL 2 has no influence on the IL 2 receptor expression on monocytes. Anti-Tac precipitates from monocyte lysates several protein bands of similar m.w. to those previously found with activated T and B cells. Finally, IFN-gamma-induced, but not resting, monocytes are found to bind recombinant IL 2. We conclude that IFN-gamma induces peripheral blood monocytes to express IL 2 receptors similar in structure to those found on activated T and B lymphocytes.
本报告描述了人外周血单核细胞上白细胞介素2（IL 2）受体的可诱导性。虽然新鲜分离的单核细胞是IL 2受体阴性，但约三分之一的细胞在培养18小时内会与抗Tac抗体发生反应。发现γ干扰素（IFN -γ）可使阳性细胞数量和结合位点数量都增加一倍，而IL 2对单核细胞上IL 2受体的表达没有影响。抗Tac从单核细胞裂解物中沉淀出几条分子量与先前在活化的T和B细胞中发现的相似的蛋白带。最后，发现经IFN -γ诱导而非静止的单核细胞能结合重组IL 2。我们得出结论，IFN -γ诱导外周血单核细胞表达结构上与活化的T和B淋巴细胞上发现的IL 2受体相似的受体。
