MacPherson G G, Fossum S, Harrison B
Sir William Dunn School of Pathology, Oxford, U.K.
Immunology. 1989 Sep;68(1):108-13.
Fresh lymph-borne (veiled) dendritic cells (L-DC) in the rat are almost totally negative for the interleukin-2 (IL-2) receptor detected by the monoclonal antibody (mAb) MRC OX39. After 16 hr culture more than 90% of L-DC are OX39 positive, and increased levels of expression can be seen within 5 hr culture. In cultures of L-DC and allogeneic lymphocytes. L-DC appear to express the IL-2 receptor more rapidly than lymphocytes. The intensity of labelling of L-DC is variable but maximal levels are similar to those seen on lymphoblasts. Culture in the presence of concanavalin A (Con A)-stimulated spleen cell supernatants or recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF) results in a more rapid and intense expression of the IL-2 receptor by L-DC. L-DC cultured following rigorous T-cell depletion, or derived from athymic rats also express the IL-2 receptor after culture with GM-CSF. Cultured, but not fresh, L-DC bind iodinated recombinant IL-2 in a dose-dependent manner and binding is inhibited by excess unlabelled ligand. The amount of IL-2 bound varies but maximal amounts are similar to those bound by lymphoblasts. Following intravenous endotoxin injection, a large proportion of freshly collected L-DC express the IL-2 receptor and the number of L-DC released into the lymph is increased. An antibody to the IL-2 receptor which blocks an allogeneic MLR has no effect on a xenogeneic MLR using rat L-DC as stimulators and mouse lymphocytes as responders.
大鼠体内新鲜的经淋巴转运的（面纱样）树突状细胞（L-DC），用单克隆抗体（mAb）MRC OX39检测时，白细胞介素-2（IL-2）受体几乎完全呈阴性。培养16小时后，超过90%的L-DC呈OX39阳性，且在培养5小时内就能观察到表达水平升高。在L-DC与同种异体淋巴细胞的共培养中，L-DC似乎比淋巴细胞更快表达IL-2受体。L-DC的标记强度各不相同，但最高水平与淋巴母细胞上所见相似。在伴刀豆球蛋白A（Con A）刺激的脾细胞上清液或重组粒细胞-巨噬细胞集落刺激因子（GM-CSF）存在的情况下进行培养，会使L-DC更快且更强烈地表达IL-2受体。经过严格T细胞去除后培养的L-DC，或源自无胸腺大鼠的L-DC，在用GM-CSF培养后也表达IL-2受体。培养后的L-DC（而非新鲜的L-DC）以剂量依赖方式结合碘化重组IL-2，且结合会被过量未标记配体抑制。结合的IL-2量各不相同，但最大量与淋巴母细胞结合的量相似。静脉注射内毒素后，很大一部分 freshly collected L-DC表达IL-2受体，且释放到淋巴中的L-DC数量增加。一种能阻断同种异体混合淋巴细胞反应（MLR）的IL-2受体抗体，对以大鼠L-DC为刺激细胞、小鼠淋巴细胞为反应细胞的异种MLR没有影响。 （注：“freshly collected”在原文中不太符合语境，推测可能是“新鲜采集的”意思，但此处结合上下文翻译可能不太准确，原文可能存在表述问题。）
