Cheung N K, Von Hoff D D, Strandjord S E, Coccia P F
J Clin Oncol. 1986 Mar;4(3):363-9. doi: 10.1200/JCO.1986.4.3.363.
Three murine monoclonal antibodies (Mab) against a cell surface antigen, the disialoganglioside GD2, were investigated for detecting bone marrow metastasis in patients with neuroblastoma (NB) by indirect immunofluorescence (IF). As few as 0.01% NB cells could be detected. No Mab reactivity was found in 60 non-NB marrows. Thirty-five marrows from patients with stage III and stage IV NB at diagnosis were examined during the course of their disease. Tumor involvement was found in 74% by Mab, 55% by the two-layer soft agar clonogenic assay (CA), and 27% by conventional histochemical stains. All marrows containing NB histologically were positive for tumor by Mab; 71% were also positive by CA. Of histologically negative marrows, 63% were Mab positive, the majority (78%) of which were also positive by CA. All Mab nonreactive samples were negative histologically and by CA. We conclude that these antibodies are highly sensitive and specific in detecting NB metastasis in bone marrow.
研究了三种针对细胞表面抗原二唾液酸神经节苷脂GD2的鼠单克隆抗体（Mab），通过间接免疫荧光法（IF）检测神经母细胞瘤（NB）患者的骨髓转移情况。低至0.01%的NB细胞都能被检测到。在60份非NB骨髓中未发现Mab反应性。对35例诊断为III期和IV期NB患者的骨髓在病程中进行了检查。通过Mab检测发现74%有肿瘤累及，通过双层软琼脂克隆形成试验（CA）检测为55%，通过传统组织化学染色检测为27%。所有组织学上含有NB的骨髓通过Mab检测肿瘤呈阳性；71%通过CA检测也呈阳性。在组织学阴性的骨髓中，63%通过Mab检测呈阳性，其中大多数（78%）通过CA检测也呈阳性。所有Mab无反应性的样本在组织学和CA检测中均为阴性。我们得出结论，这些抗体在检测NB骨髓转移方面具有高度敏感性和特异性。
