1st Department of Pathology and Experimental Cancer Research.
Brain Metastasis Research Group, Hungarian Academy of Sciences, 2nd Department of Pathology, Semmelweis University, Budapest, Hungary.
J Neuropathol Exp Neurol. 2019 Apr 1;78(4):326-339. doi: 10.1093/jnen/nlz007.
The impact of growth pattern on the distribution of connective tissue and on the vascularization of brain metastases (40 colon, lung and breast carcinoma samples) was analyzed. Most of the cases showed either a "pushing-type" (18/40, mostly colon and lung carcinomas) or a "papillary-type" (19/40, mostly breast carcinomas) growth pattern. There was a striking difference in the growth pattern and vascularization of colon/lung versus breast carcinoma metastases. Pushing-type brain metastases incorporated fewer vessels and accumulated more collagen in the adjacent brain parenchyma, whereas papillary-type brain metastases incorporated more vessels and accumulated collagen in the center of the tumor. We observed duplication of the PDGFRβ-positive pericyte layer accompanied by an increase in the amount of collagen within the vessel walls. The outer layer of pericytes and the collagen was removed from the vessel by invasive activity of the tumors, which occurred either peri- or intratumorally, depending on the growth pattern of the metastasis. Our findings suggest that pericytes are the main source of the connective tissue in brain metastases. Vascularization and connective tissue accumulation of the brain metastases largely depend on the growth pattern of the tumors.
我们分析了生长模式对脑转移瘤（40 例结肠癌、肺癌和乳腺癌样本）中结缔组织分布和血管生成的影响。大多数病例表现出“推挤型”（18/40，主要为结肠癌和肺癌）或“乳头状型”（19/40，主要为乳腺癌）生长模式。结肠癌/肺癌与乳腺癌转移瘤的生长模式和血管生成存在显著差异。推挤型脑转移瘤在邻近脑实质中包含较少的血管，并积累更多的胶原，而乳头状型脑转移瘤在肿瘤中心包含更多的血管并积累胶原。我们观察到 PDGFRβ 阳性周细胞层的复制，伴随血管壁内胶原含量的增加。周细胞的外层和胶原被肿瘤的侵袭活性从血管中去除，这取决于转移瘤的生长模式，是发生在肿瘤周围还是肿瘤内。我们的研究结果表明，周细胞是脑转移瘤中结缔组织的主要来源。脑转移瘤的血管生成和结缔组织积累在很大程度上取决于肿瘤的生长模式。
