阿非科林对JU56细胞不同效应之间的剂量关系。

Dose relationships between different effects of aphidicolin in JU56 cells.

作者信息

Moore R C, Randell C, Bender M A

出版信息

Mutat Res. 1986 Apr;160(2):103-9. doi: 10.1016/0027-5107(86)90034-5.

Abstract

Some effects of aphidicolin have been investigated in relationship to dose, in a permanent cell line, JU56. Inhibition of semi-conservative DNA synthesis occurred at concentrations greater than 3 X 10(-7) M. In this respect the cells were about as sensitive as L1210 and HeLa cells, and more than 10-fold more sensitive than PHA-stimulated human peripheral blood leucocytes. Delay of progress of cells through G2 occurred at concentrations which inhibited synthesis to about 2% of control levels. Chromatid aberrations appeared in cells at concentrations which decreased synthesis to 5%. Synergism with X-rays in the production of chromatid aberrations occurred at doses which reduced semi-conservative synthesis to 40% of control levels. Isochromatid aberrations appeared in cells continuously exposed to aphidicolin in G2 at concentrations which reduced synthesis to 5% of control units.

摘要

在永久性细胞系JU56中，已研究了阿非科林与剂量相关的一些效应。当浓度大于3×10⁻⁷ M时，半保留DNA合成受到抑制。在这方面，这些细胞的敏感性与L1210细胞和HeLa细胞相当，比植物血凝素刺激的人外周血白细胞敏感10倍以上。当合成被抑制至对照水平的约2%时，细胞通过G2期的进程会延迟。当合成降至5%时，细胞中出现染色单体畸变。在将半保留合成降至对照水平的40%的剂量下，与X射线在产生染色单体畸变方面存在协同作用。在G2期持续暴露于阿非科林的细胞中，当合成降至对照单位的5%时，出现等染色单体畸变。