Department of Biotechnology, College of Life Science, Shanxi Agricultural University, Taigu, Shanxi 030801, China; Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
Shanxi Key Laboratory of Ecological Animal Science and Environmental Veterinary Medicine, College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, China.
Chemosphere. 2019 Jun;224:71-76. doi: 10.1016/j.chemosphere.2019.02.113. Epub 2019 Feb 18.
Fluoride is one of the common environmental pollutants. Internal exposure to fluoride is related to the lowered cognitive function and intelligence, particularly for children. Determination of protein content in brain tissue is a means to reflect the functional development of the central nervous system. Insulin and insulin receptor (IR) signaling systems are associated with cognitive ability. The present research focused on the assessment of the expressions of IR protein and mRNA in hippocampus and olfactory bulb (OB), as well as learning and memory ability of male Kunming mice. Mice were exposed to 50, 100, and 150 mg/L NaF for 90 continuous days. The results showed that learning and memory abilities as well as protein content of male mice brain was significantly decreased by fluoride. Fluoride could inhibit the protein and mRNA expressions of the IR in the hippocampus and OB of mice. IRs mainly distributed in the olfactory nerve layer of the outermost layer of the OB, and most distributed in the hippocampal cornu ammon 3 (CA3) region, followed by the dentate gyrus (DG) and cornu ammon 1 (CA1) regions. These findings suggested that inhibition of the IR protein and mRNA expressions in the hippocampus and OB by fluoride might in part affect learning and memory ability in male mice.
氟化物是常见的环境污染物之一。内源性氟暴露与认知功能和智力下降有关，尤其是儿童。脑组织蛋白含量的测定是反映中枢神经系统功能发育的一种手段。胰岛素和胰岛素受体（IR）信号系统与认知能力有关。本研究重点评估了雄性昆明小鼠海马和嗅球（OB）中 IR 蛋白和 mRNA 的表达，以及学习和记忆能力。将小鼠暴露于 50、100 和 150mg/L 的 NaF 中 90 天。结果表明，氟化物可显著降低雄性小鼠的学习和记忆能力以及大脑中的蛋白质含量。氟化物可抑制海马和 OB 中 IR 的蛋白和 mRNA 表达。IR 主要分布在 OB 最外层的嗅神经层，在海马角 3（CA3）区分布最多，其次是齿状回（DG）和海马角 1（CA1）区。这些发现表明，氟化物抑制海马和 OB 中 IR 蛋白和 mRNA 的表达可能部分影响雄性小鼠的学习和记忆能力。
