a National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology , Inage-ku , Chiba , Japan.
b National Center for Geriatrics and Gerontology , Obu , Aichi , Japan.
Int J Radiat Biol. 2019 Aug;95(8):1144-1149. doi: 10.1080/09553002.2019.1589019. Epub 2019 Mar 18.
The aim of this study was to establish a new method of real-time, in vivo detection of radiation damage and recovery. The thymus was observed under fluorescent light in a green fluorescent protein transgenic medaka. After irradiation, medaka thymus images were analyzed to quantify the effects of radiation by measuring changes in thymus size. A single acute irradiation of X-rays (0-30 Gy) or heavy Fe ions (0-10 Gy) was delivered to the medaka. Images were captured 0, 1, 2, 3, 5, 7, 11, and 21 d after irradiation. Dose-response assessment was conducted to provide a direct measurement of the effects of the radiation. A biomonitoring system to detect the effects of radiation in real time was established. Using this system, the threshold doses for the induction of thymic atrophy by acute X-rays and Fe ions were 2-5 Gy and 0.5-1 Gy, respectively. The Relative Biological Effectiveness (RBE) of Fe-ion to X-rays was estimated to be around 3. This system may be used to evaluate the risk from concurrent exposure to hazards, such as chemicals and radiation, and for aging research.
本研究旨在建立一种实时、体内检测辐射损伤和恢复的新方法。在绿色荧光蛋白转基因斑马鱼中观察荧光下的胸腺。照射后，通过测量胸腺大小的变化来分析斑马鱼胸腺图像，以定量评估辐射的影响。单次急性 X 射线（0-30Gy）或重铁离子（0-10Gy）照射到斑马鱼。在照射后 0、1、2、3、5、7、11 和 21 天拍摄图像。进行剂量反应评估，以提供辐射影响的直接测量。建立了一个实时检测辐射影响的生物监测系统。使用该系统，急性 X 射线和铁离子诱导胸腺萎缩的阈值剂量分别为 2-5Gy 和 0.5-1Gy。Fe 离子对 X 射线的相对生物效应（RBE）估计约为 3。该系统可用于评估同时暴露于化学物质和辐射等危害的风险，以及用于衰老研究。
