Li Jia, Zhao Yan, Huang Caoxin, Chen Zheng, Shi Xiulin, Li Long, Chen Zhong, Li Xuejun
Xiamen Diabetes Institute, The First Affiliated Hospital of Xiamen University, Xiamen, China.
Department of Electronic Science, State Key Laboratory of Physical Chemistry of Solid Surfaces, Xiamen University, Xiamen, China.
Endocr Connect. 2019 Apr;8(4):299-308. doi: 10.1530/EC-19-0023.
Exercise benefits people with nonalcoholic fatty liver disease (NAFLD). The aim of this study was to identify a panel of biomarkers and to provide the possible mechanism for the effect of exercise on NAFLD patients via an untargeted mass spectrometry-based serum metabolomics study.
NAFLD patients were classified randomly into a control group (n = 74) and a 6-month vigorous exercise (n = 68) group. Differences in serum metabolic profiles were analyzed using untargeted ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) technology. Principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were used to validate the differences between these two groups, and altered metabolites were obtained by ANOVA (fold change >2, P < 0.05) and identified with the online database Metlin and an in-house database.
Metabolic profiling and multiple statistical analyses of the serum samples indicated significant differences between the NAFLD patients in the control and the 6-month vigorous exercise groups. Finally, 36 metabolites were identified between the control vs exercise groups. These metabolites were mainly associated with glycerophospholipid- and sphingolipid-related pathways.
Our study demonstrates that glycerophospholipid and sphingolipid alterations may contribute to the mechanism underlying the effect of exercise on NAFLD patients. A LC-MS-based metabolomics approach has a potential value for screening exercise-induced biomarkers.
运动对非酒精性脂肪性肝病（NAFLD）患者有益。本研究旨在通过基于非靶向质谱的血清代谢组学研究，确定一组生物标志物，并提供运动对NAFLD患者影响的可能机制。
将NAFLD患者随机分为对照组（n = 74）和6个月剧烈运动组（n = 68）。采用非靶向超高效液相色谱-四极杆飞行时间质谱联用（UPLC-Q-TOF/MS）技术分析血清代谢谱差异。主成分分析（PCA）和正交偏最小二乘法判别分析（OPLS-DA）用于验证两组之间的差异，并通过方差分析（变化倍数>2，P < 0.05）获得改变的代谢物，并使用在线数据库Metlin和内部数据库进行鉴定。
血清样本的代谢谱分析和多种统计分析表明，对照组和6个月剧烈运动组的NAFLD患者之间存在显著差异。最终，在对照组与运动组之间鉴定出36种代谢物。这些代谢物主要与甘油磷脂和鞘脂相关途径有关。
我们的研究表明，甘油磷脂和鞘脂的改变可能有助于解释运动对NAFLD患者影响的潜在机制。基于液相色谱-质谱联用的代谢组学方法在筛选运动诱导的生物标志物方面具有潜在价值。
