通过代谢工程增强产丁二酸梭菌的酸再吸收和生物溶剂生产能力，以从木质纤维素生物质高效生产生物燃料。
Enhancement of acid re-assimilation and biosolvent production in Clostridium saccharoperbutylacetonicum through metabolic engineering for efficient biofuel production from lignocellulosic biomass.
机构信息
Department of Biosystems Engineering, Auburn University, Auburn, AL 36849, USA.
College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.
出版信息
Bioresour Technol. 2019 Jun;281:217-225. doi: 10.1016/j.biortech.2019.02.096. Epub 2019 Feb 23.
In the clostridial acetone-butanol-ethanol (ABE) fermentation, the intermediate acetate and butyrate are re-assimilated for solvent production. Here, key genes in ABE pathways in Clostridium saccharoperbutylacetonicum N1-4 were overexpressed to enhance acid re-assimilation and solvent production. With the overexpression of sol operon, acid re-assimilation was enhanced, and ABE production was increased by 20%, with ethanol production increased by six times but almost no increase in butanol production. To further drive carbon flux for C4 metabolites and ultimate butanol production, key genes including hbd, thl, crt and bcd in butanol production pathway were further overexpressed. Compared to the control, butanol, acetone and total ABE production in the new strain was increased by 8%, 18%, and 12.4%, respectively. Finally, simultaneous saccharification and fermentation was carried out using acetate-pretreated switchgrass. 15.4 g/L total ABE (with a yield of 0.31 g/g) was produced in both engineered strains, which was significantly higher than the control.
在梭菌丙酮丁醇乙醇（ABE）发酵中，中间产物乙酸盐和丁酸盐被重新用于溶剂生产。在这里，过表达了丙酮丁醇途径中的关键基因，以增强酸的再同化和溶剂的生产。通过过表达 sol 操纵子，增强了酸的再同化，ABE 的产量增加了 20%，乙醇的产量增加了六倍，但丁醇的产量几乎没有增加。为了进一步推动 C4 代谢物和最终丁醇的产生的碳通量，进一步过表达了丁醇生产途径中的关键基因，包括 hbd、thl、crt 和 bcd。与对照相比，新菌株中的丁醇、丙酮和总 ABE 产量分别提高了 8%、18%和 12.4%。最后，使用乙酸预处理的柳枝稷进行同步糖化发酵。在两个工程菌株中都产生了 15.4 g/L 的总 ABE（产率为 0.31 g/g），明显高于对照。
Zotero 插件