一种筛选葡萄种质对南方根结线虫抗性的技术。

A Technique for Screening Grape Germplasm for Resistance to Meloidogyne incognita.

作者信息

Cousins P M, Walker M A

机构信息

USDA-ARS, Plant Genetic Resources Unit, New York State Agricultural Experiment Station, Cornell University, Geneva 14456.

Department of Viticulture and Enology, University of California, Davis 95616.

出版信息

Plant Dis. 2001 Oct;85(10):1052-1054. doi: 10.1094/PDIS.2001.85.10.1052.

DOI:10.1094/PDIS.2001.85.10.1052

PMID:30823275

Abstract

A technique to evaluate the root-knot nematode resistance of grape seedlings was developed. Seedlings of rootstock crosses and nematode-susceptible Vitis vinifera cvs. Colombard and Carignane were inoculated with Meloidogyne incognita juveniles. Reproduction of nematodes on individual plants was measured by counting the number of egg masses stained with eosin and the number of eggs present. Egg mass counts were highly correlated with egg counts. Resistant and susceptible cultivars could be clearly distinguished by the number of egg masses produced on vegetatively propagated cuttings. It is concluded that egg mass counting can substitute for the more laborious and time-consuming methods of counting nematode eggs or juveniles in the evaluation of root-knot nematode resistance in Vitis.

摘要

开发了一种评估葡萄幼苗根结线虫抗性的技术。砧木杂交种和对线虫敏感的酿酒葡萄品种科隆巴德和佳丽酿的幼苗接种南方根结线虫幼虫。通过计数用伊红染色的卵块数量和存在的卵数量来测量线虫在单株植物上的繁殖情况。卵块计数与卵计数高度相关。通过营养繁殖插条上产生的卵块数量可以清楚地区分抗性和敏感品种。得出结论，在评估葡萄根结线虫抗性时，卵块计数可以替代计数线虫卵或幼虫这种更费力且耗时的方法。